The Substrate Specificity of Adenosine 3':5'-cyclic Monophosphate-dependent Protein Kinase of Rabbit Skeletal Muscle

Overview

Journal Biochem J

Specialty Biochemistry

Date 1977 Feb 15

PMID 192223

Citations 14

Authors

S J Yeaman

P Cohen

D C Watson

G H DIXON

Affiliations

Soon will be listed here.

Abstract

The known amino acid sequences at the two sites on phosphorylase kinase that are phosphorylated by cyclic AMP-dependent protein kinase were extended. The sequences of 42 amino acids around the phosphorylation site on the alpha-subunit and of 14 amino acids around the phosphorylation site on the beta-subunit were shown to be: alpha-subunit Phe-Arg-Arg-Leu-Ser(P)-Ile-Ser-Thr-Glu-Ser-Glx-Pro-Asx-Gly-Gly-His-Ser-Leu-Gly-Ala-Asp-Leu-Met-Ser-Pro-Ser-Phe-Leu-Ser-Pro-Gly-Thr-Ser-Val-Phe(Ser,Pro,Gly)His-Thr-Ser-Lys; beta-subunit, Ala-Arg-Thr-Lys-Arg-Ser-Gly-Ser(P)-VALIle-Tyr-Glu-Pro-Leu-Lys. The sites on histone H2B which are phosphorylated by cyclic AMP-dependent protein kinase in vitro were identified as serine-36 and serine-32. The amino acid sequence in this region is: Lys-Lys-Arg-Lys-Arg-Ser32(P)-Arg-Lys-Glu-Ser36(P)-Tyr-Ser-Val-Tyr-Val- [Iwai, K., Ishikawa, K. & Hayashi, H. (1970) Nature (London) 226, 1056-1058]. Serine-36 was phosphorylated at 50% of the rate at which the beta-subunit of phosphorylase kinase was phosphorylated, and it was phosphorylated 6-7-fold more rapidly than was serine-32. The amino acid sequences when compared with those at the phosphorylation sites of other physiological substrates suggest that the presence of two adjacent basic amino acids on the N-terminal side of the susceptible serine residue may be critical for specific substrate recognition in vivo.

Citing Articles

Protein kinase A catalytic subunit isoform PRKACA; History, function and physiology.

Turnham R, Scott J Gene. 2015; 577(2):101-8.

PMID: 26687711 PMC: 4713328. DOI: 10.1016/j.gene.2015.11.052.

Identification of autophosphorylation sites in eukaryotic elongation factor-2 kinase.

Pyr Dit Ruys S, Wang X, Smith E, Herinckx G, Hussain N, Rider M Biochem J. 2012; 442(3):681-92.

PMID: 22216903 PMC: 3286862. DOI: 10.1042/BJ20111530.

Interaction sites on phosphorylase kinase for calmodulin.

Heilmeyer Jr L, Gerschinski A, Meyer H, Jennissen H Mol Cell Biochem. 1993; 127-128:19-30.

PMID: 7935351 DOI: 10.1007/BF01076754.

Analysis of desmin and vimentin phosphopeptides in cultured avian myogenic cells and their modulation by 8-bromo-adenosine 3',5'-cyclic monophosphate.

Gard D, Lazarides E Proc Natl Acad Sci U S A. 1982; 79(22):6912-6.

PMID: 6294666 PMC: 347244. DOI: 10.1073/pnas.79.22.6912.

cDNA clone coding for part of a mouse H-2d major histocompatibility antigen.

Kvist S, Bregegere F, Rask L, Cami B, Garoff H, Daniel F Proc Natl Acad Sci U S A. 1981; 78(5):2772-6.

PMID: 6265910 PMC: 319439. DOI: 10.1073/pnas.78.5.2772.

References

Kemp B, Bylund D, Huang T, KREBS E . Substrate specificity of the cyclic AMP-dependent protein kinase. Proc Natl Acad Sci U S A. 1975; 72(9):3448-52. PMC: 433011. DOI: 10.1073/pnas.72.9.3448. View

Edlund B, Andersson J, Titanji V, Dahlqvist U, Ekman P, ZETTERQVIST O . Amino acid sequence at the phosphorylated site of rat liver pyruvate kinase. Biochem Biophys Res Commun. 1975; 67(4):1516-21. DOI: 10.1016/0006-291x(75)90198-9. View

Yeaman S, Cohen P . The hormonal control of activity of skeletal muscle phosphorylase kinase. Phosphorylation of the enzyme at two sites in vivo in response to adrenalin. Eur J Biochem. 1975; 51(1):93-104. DOI: 10.1111/j.1432-1033.1975.tb03910.x. View

Shlyapnikov S, Arutyunyan A, Kurochkin S, Memelova L, Nesterova M, Sashchenko L . Investigation of the sites phosphorylated in lysine-rich histones by protein kinase from pig brain. FEBS Lett. 1975; 53(3):316-9. DOI: 10.1016/0014-5793(75)80045-7. View

Cohen P, Watson D, DIXON G . The hormonal control of activity of skeletal muscle phosphorylase kinase. Amino-acid sequences at the two sites of action of adenosine-3':5'-monophosphate-dependent protein kinase. Eur J Biochem. 1975; 51(1):79-92. DOI: 10.1111/j.1432-1033.1975.tb03909.x. View

Bylund D, KREBS E . Effect of denaturation on the susceptibility of proteins to enzymic phosphorylation. J Biol Chem. 1975; 250(16):6355-61. View

Daile P, Carnegie P, Young J . Synthetic substrate for cyclic AMP-dependent protein kinase. Nature. 1975; 257(5525):416-8. DOI: 10.1038/257416a0. View

Hashimoto E, Takeda M, NISHIZUKA Y . Phosphorylated sites of calf thymus histone H2B by adenosine 3',5'-monophosphate-dependent protein kinase from silkworm. Biochem Biophys Res Commun. 1975; 66(2):547-55. DOI: 10.1016/0006-291x(75)90545-8. View

Humble E, Berglund L, Titanji V, Ljungstrom O, Edlund B, ZETTERQVIST O . Non-dependence on native structure of pig liver pyruvate kinase when used as a substrate for cyclic 3',5'-AMP-stimulated protein kinase. Biochem Biophys Res Commun. 1975; 66(2):614-21. DOI: 10.1016/0006-291x(75)90554-9. View

10.

Kemp B, Benjamini E, KREBS E . Synthetic hexapeptide substrates and inhibitors of 3':5'-cyclic AMP-dependent protein kinase. Proc Natl Acad Sci U S A. 1976; 73(4):1038-42. PMC: 430195. DOI: 10.1073/pnas.73.4.1038. View

11.

ZETTERQVIST O, Ragnarsson U, Humble E, Berglund L, Engstrom L . The minimum substrate of cyclic AMP-stimulated protein kinase, as studied by synthetic peptides representing the phosphorylatable site of pyruvate kinase (type L) of rat liver. Biochem Biophys Res Commun. 1976; 70(3):696-703. DOI: 10.1016/0006-291x(76)90648-3. View

12.

Nimmo H, Proud C, Cohen P . The phosphorylation of rabbit skeletal muscle glycogen synthase by glycogen synthase kinase-2 and adenosine-3':5'-monophosphate-dependent protein kinase. Eur J Biochem. 1976; 68(1):31-44. DOI: 10.1111/j.1432-1033.1976.tb10762.x. View

13.

Daile P, Carnegie P . Peptides from myelin basic protein as substrates for adenosine 3', 5'-cyclic monophosphate-dependent protein kinases. Biochem Biophys Res Commun. 1974; 61(3):852-8. DOI: 10.1016/0006-291x(74)90234-4. View

14.

LANGAN T . Phosphorylation of liver histone following the administration of glucagon and insulin. Proc Natl Acad Sci U S A. 1969; 64(4):1276-83. PMC: 223280. DOI: 10.1073/pnas.64.4.1276. View

15.

Walsh D, Ashby C, Gonzalez C, Calkins D, FISCHER E . Krebs EG: Purification and characterization of a protein inhibitor of adenosine 3',5'-monophosphate-dependent protein kinases. J Biol Chem. 1971; 246(7):1977-85. View

16.

LANGAN T . Cyclic AMP and histone phosphorylation. Ann N Y Acad Sci. 1971; 185:166-80. DOI: 10.1111/j.1749-6632.1971.tb45246.x. View

17.

Cohen P . The subunit structure of rabbit-skeletal-muscle phosphorylase kinase, and the molecular basis of its activation reactions. Eur J Biochem. 1973; 34(1):1-14. DOI: 10.1111/j.1432-1033.1973.tb02721.x. View

18.

Hjelmquist G, Andersson J, Edlund B, Engstroom L . Amino acid sequence of a (32P) phosphopeptide from pig liver pyruvate kinase phosphorylated by cyclic 3',5'-AMP-stimulated protein kinase and gamma-(32P)ATP. Biochem Biophys Res Commun. 1974; 61(2):559-63. DOI: 10.1016/0006-291x(74)90993-0. View

19.

Sanders M, DIXON G . The biosynthesis of protamine in trout testis. IV. Sites of phosphorylation. J Biol Chem. 1972; 247(3):851-5. View

20.

Marzluff Jr W, McCarty K . Structural studies of calf thymus F3 histone. II. Occurrence of phosphoserine and -N-acetyllysine in thermolysin peptides. Biochemistry. 1972; 11(14):2677-81. DOI: 10.1021/bi00764a020. View