Control of Arabidopsis Meristem Development by Thioredoxin-dependent Regulation of Intercellular Transport

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2009 Feb 17

PMID 19218459

Citations 107

Authors

Yoselin Benitez-Alfonso

Michelle Cilia

Adrianna San Roman

Carole Thomas

Andy Maule

Stephen Hearn

David Jackson

Affiliations

Soon will be listed here.

Abstract

Cell-to-cell transport in plants occurs through cytoplasmic channels called "plasmodesmata" and is regulated by developmental and environmental factors. Callose deposition modulates plasmodesmal transport in vivo, but little is known about the mechanisms that regulate this process. Here we report a genetic approach to identify mutants affecting plasmodesmal transport. We isolated 5 mutants, named gfp arrested trafficking (gat), affected in GFP unloading from the phloem into the meristem. gat1 mutants were seedling lethal and carried lesions in an m-type thioredoxin that is expressed in non-green plastids of meristems and organ primordia. Callose and hydrogen peroxide accumulated in gat1 mutants, and WT plants subjected to oxidative conditions phenocopied the gat1 trafficking defects. Ectopic expression of GAT1 in mature leaves increased plasmodesmal permeability and led to a delay in senescence and flowering time. We propose a role for the GAT1 thioredoxin in the redox regulation of callose deposition and symplastic permeability that is essential for meristem maintenance in Arabidopsis.

Citing Articles

Communicating Across Cell Walls: Structure, Evolution, and Regulation of Plasmodesmatal Transport in Plants.

Brunkard J Results Probl Cell Differ. 2024; 73:73-86.

PMID: 39242375 DOI: 10.1007/978-3-031-62036-2_4.

Molecular identification of two thioredoxin genes and their function in antioxidant defense in diapause.

Shen Z, Luo Q, Mao J, Li Y, Wang M, Zhang L Front Physiol. 2024; 15:1440531.

PMID: 39113938 PMC: 11303210. DOI: 10.3389/fphys.2024.1440531.

Distributing Plant Developmental Regulatory Proteins via Plasmodesmata.

Schreiber J, Limpens E, de Keijzer J Plants (Basel). 2024; 13(5).

PMID: 38475529 PMC: 10933835. DOI: 10.3390/plants13050684.

Plasmodesmal connectivity in C Gynandropsis gynandra is induced by light and dependent on photosynthesis.

Schreier T, Muller K, Eicke S, Faulkner C, Zeeman S, Hibberd J New Phytol. 2023; 241(1):298-313.

PMID: 37882365 PMC: 10952754. DOI: 10.1111/nph.19343.

Thioredoxins m regulate plastid glucose-6-phosphate dehydrogenase activity in Arabidopsis roots under salt stress.

Nee G, Wang F, Chatel-Innocenti G, Mhamdi A, Juranville E, Vanacker H Front Plant Sci. 2023; 14:1179112.

PMID: 37332692 PMC: 10274509. DOI: 10.3389/fpls.2023.1179112.

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