B6D2F1 Mice Are a Suitable Model of Oxidative Stress-mediated Impaired Endothelium-dependent Dilation with Aging

Overview

Journal J Gerontol A Biol Sci Med Sci

Specialty Geriatrics

Date 2009 Feb 13

PMID 19211548

Citations 63

Authors

Lisa A Lesniewski

Melanie L Connell

Jessica R Durrant

Brian J Folian

Martin C Anderson

Anthony J Donato

Douglas R Seals

Affiliations

Soon will be listed here.

Abstract

To determine if B6D2F1 mice represent a suitable model of oxidative stress-mediated impaired endothelium-dependent dilation (EDD) with aging, mice were studied at 6.9 +/- 0.3 and 31.9 +/- 0.6 months. EDD to acetylcholine (ACh) was 26% (p < .001) and 12% (p < .001) lower, respectively, in isolated carotid (n = 10-11) and femoral (n = 10) arteries from older mice, and reductions in arterial pressure to systemic ACh infusion were smaller in older mice (n = 6-10; p < .01). Nitrotyrosine was marked in aorta of older mice (p < .05, n = 4). Superoxide production in carotid arteries was greater (p < .05), and TEMPOL restored dilation in carotid arteries and systemically in older mice. N(G)-nitro-l-arginine methyl ester (l-NAME) reduced carotid artery dilation in young more than older mice, whereas TEMPOL restored the effects of l-NAME in older mice. Carotid artery stiffness was increased in older compared with young mice (p = .04). Our results provide the first comprehensive evidence that B6D2F1 mice are a useful model for investigating mechanisms of reduced nitric oxide-dependent, oxidative stress-associated EDD and increased arterial stiffness with aging.

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