Structural and Thermodynamic Behavior of Cytochrome C Assembled with Glutathione-covered Gold Nanoparticles

Overview

Journal J Biol Inorg Chem

Publisher Springer

Specialty Biochemistry

Date 2009 Feb 12

PMID 19205757

Citations 1

Authors

Eva Valusova

Peter Svec

Marian Antalik

Affiliations

Soon will be listed here.

Abstract

The conformational changes of horse heart ferricytochrome c (cyt c) after association of gold nanoparticles have been studied by electronic absorption spectroscopy and circular dichroism (CD). Our results show that the structural stability around the heme of complexed cyt c was increased successfully. Glutathione-layered gold nanoparticles caused a significant increase of the apparent pK values of the cyt c alkaline transition. Similarly, the heme crevice became more stable to heat after assembly of cyt c with gold nanoparticles. In contrast, gold nanoparticles weaken the overall thermal stability of the cyt c by decreasing the denaturation temperature estimated from far-UV CD measurements. Similar behavior has previously been reported for cyt c complexed with physiological redox partners as well as hydrophilic polyanions.

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