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Using a Commercial DiversiLab Semiautomated Repetitive Sequence-based PCR Typing Technique for Identification of Escherichia Coli Clone ST131 Producing CTX-M-15

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 2009 Feb 11
PMID 19204095
Citations 28
Authors
Johann D D Pitout
Lorraine Campbell
Deirdre L Church
Pauline W Wang
David S Guttman
Daniel B Gregson
Affiliations
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Abstract

A study was designed to evaluate the ability of the DiversiLab fingerprinting kit, a type of repetitive element PCR (rep-PCR), to identify Escherichia coli clone ST131 producing beta-lactamase CTX-M-15. A set of 53 nonduplicate isolates of extended-spectrum beta-lactamase-producing E. coli underwent rep-PCR, pulsed-field gel electrophoresis, and multilocus sequence typing. The DiversiLab system successfully identified E. coli clone ST131 producing CTX-M-15 and provides a simple standardized typing protocol for monitoring the spread of this clone.

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References
1.
Hunter S, Vauterin P, Lambert-Fair M, Van Duyne M, Kubota K, Graves L . Establishment of a universal size standard strain for use with the PulseNet standardized pulsed-field gel electrophoresis protocols: converting the national databases to the new size standard. J Clin Microbiol. 2005; 43(3):1045-50. PMC: 1081233. DOI: 10.1128/JCM.43.3.1045-1050.2005. View
2.
Tenover F . Rapid detection and identification of bacterial pathogens using novel molecular technologies: infection control and beyond. Clin Infect Dis. 2007; 44(3):418-23. DOI: 10.1086/510684. View
3.
Pitout J, Laupland K . Extended-spectrum beta-lactamase-producing Enterobacteriaceae: an emerging public-health concern. Lancet Infect Dis. 2008; 8(3):159-66. DOI: 10.1016/S1473-3099(08)70041-0. View
4.
Pitout J, Church D, Gregson D, Chow B, McCracken M, Mulvey M . Molecular epidemiology of CTX-M-producing Escherichia coli in the Calgary Health Region: emergence of CTX-M-15-producing isolates. Antimicrob Agents Chemother. 2007; 51(4):1281-6. PMC: 1855502. DOI: 10.1128/AAC.01377-06. View
5.
Lau S, Kaufmann M, Livermore D, Woodford N, Willshaw G, Cheasty T . UK epidemic Escherichia coli strains A-E, with CTX-M-15 beta-lactamase, all belong to the international O25:H4-ST131 clone. J Antimicrob Chemother. 2008; 62(6):1241-4. DOI: 10.1093/jac/dkn380. View