Specific DNA Binding of the TraM Protein to the OriT Region of Plasmid R100

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1991 Oct 1

PMID 1917866

Citations 16

Authors

T Abo

S Inamoto

E Ohtsubo

Affiliations

Soon will be listed here.

Abstract

The product of the traM gene of plasmid R100 was purified as the TraM-collagen-beta-galactosidase fusion protein (TraM*) by using a beta-galactosidase-specific affinity column, and the TraM portion of TraM* (TraM') was separated by collagenolysis. Both the TraM* and TraM' proteins were found to bind specifically to a broad region preceding the traM gene. This region (designated sbm) was located within the nonconserved region in oriT among conjugative plasmids related to R100. The region seems to contain four core binding sites (designated sbmA, sbmB, sbmC, and sbmD), each consisting of a similar number of nucleotides and including a homologous 15-bp sequence. This result, together with the observation that the TraM* protein was located in the membrane fraction, indicates the possibility that the TraM protein has a function in anchoring the oriT region of R100 at the sbm sites to the membrane pore, through which the single-stranded DNA is transferred to the recipient. sbmC and sbmD, each of which contained a characteristic inverted repeat sequence, overlapped with the promoter region for the traM gene. This suggests that the expression of the traM gene may be regulated by its own product.

Citing Articles

Spread and Persistence of Virulence and Antibiotic Resistance Genes: A Ride on the F Plasmid Conjugation Module.

Koraimann G EcoSal Plus. 2018; 8(1).

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Identification of oriT and a recombination hot spot in the IncA/C plasmid backbone.

Hegyi A, Szabo M, Olasz F, Kiss J Sci Rep. 2017; 7(1):10595.

PMID: 28878309 PMC: 5587640. DOI: 10.1038/s41598-017-11097-0.

Structural basis of cooperative DNA recognition by the plasmid conjugation factor, TraM.

Wong J, Lu J, Edwards R, Frost L, Glover J Nucleic Acids Res. 2011; 39(15):6775-88.

PMID: 21565799 PMC: 3159463. DOI: 10.1093/nar/gkr296.

The F-plasmid TraI protein contains three functional domains required for conjugative DNA strand transfer.

Matson S, Ragonese H J Bacteriol. 2005; 187(2):697-706.

PMID: 15629940 PMC: 543555. DOI: 10.1128/JB.187.2.697-706.2005.

Thirty-eight C-terminal amino acids of the coupling protein TraD of the F-like conjugative resistance plasmid R1 are required and sufficient to confer binding to the substrate selector protein TraM.

Beranek A, Zettl M, Lorenzoni K, Schauer A, Manhart M, Koraimann G J Bacteriol. 2004; 186(20):6999-7006.

PMID: 15466052 PMC: 522193. DOI: 10.1128/JB.186.20.6999-7006.2004.

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