Effect of Synthetic Porcine Neuropeptide Y (NPY) on Splanchnic Blood Flows and Exocrine Pancreatic Secretion in Dogs

Overview

Journal Dig Dis Sci

Specialty Gastroenterology

Date 2009 Jan 24

PMID 19160602

Citations 1

Authors

S Sumi

K Inoue

M Kogire

R Doi

M Yun

H Kaji

R Hosotani

M Fujimura

K Uchida

S Kiyama

K Kitagawa

H Yajima

N Fujii

T Tobe

Affiliations

Soon will be listed here.

Abstract

This study examined the effect of synthetic porcine neuropeptide Y on the splanchnic blood flows and the exocrine pancreatic secretion in dogs. Graded doses of neuropeptide Y (0.1-5 microg/kg, intravenous) caused dose-dependent reduction of the secretin-stimulated exocrine pancreatic secretion and of the blood flows in the superior mesenteric artery, the portal vein, and the pancreatic tissue. Neuropeptide Y at 5 microg/kg reduced the blood flows to 45.9 +/- 13.3% (superior mesenteric artery), 63.0 +/- 10.5% (portal vein), and 77.9 +/- 4.8% (pancreatic tissue), respectively. This dose also reduced secretin-stimulated pancreatic juice volume and CCK-8 plus secretin-stimulated protein output to 65.2 +/- 9.3 and 63.3 +/- 14.0%, respectively. This study shows a potent vasoconstrictor effect of neuropeptide Y on splanchnic vessels. Neuropeptide Y also inhibited exocrine pancreatic secretion in a significant correlation with the reduction in pancreatic tissue blood flow, which suggests that reduction in the blood flow may be one of the possible mechanisms of the inhibitory action of neuropeptide Y on exocrine secretion.

Citing Articles

Overview of exocrine pancreatic pathobiology.

Pandiri A Toxicol Pathol. 2013; 42(1):207-16.

PMID: 24190915 PMC: 4360889. DOI: 10.1177/0192623313509907.

References

Inoue K, Kawano T, Shima K, Suzuki T, Tobe T, Yajima H . Relationship between development of fibrosis and hemodynamic changes of the pancreas in dogs. Gastroenterology. 1981; 81(1):37-47. View

Jung G, Louie D, Owyang C . Pancreatic polypeptide inhibits pancreatic enzyme secretion via a cholinergic pathway. Am J Physiol. 1987; 253(5 Pt 1):G706-10. DOI: 10.1152/ajpgi.1987.253.5.G706. View

Sumi S, Inoue K, Kogire M, Doi R, Takaori K, Yajima H . Effect of synthetic neuromedin-N, a novel neurotensin-like peptide, on exocrine pancreatic secretion and splanchnic blood flow in dogs. Neuropeptides. 1987; 9(3):247-55. DOI: 10.1016/0143-4179(87)90045-x. View

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

Hellstrom P, Olerup O, Tatemoto K . Neuropeptide Y may mediate effects of sympathetic nerve stimulations on colonic motility and blood flow in the cat. Acta Physiol Scand. 1985; 124(4):613-24. DOI: 10.1111/j.1748-1716.1985.tb00055.x. View

Inoue K, Hosotani R, Tatemoto K, Yajima H, Tobe T . Effect of natural peptide YY on blood flow and exocrine secretion of pancreas in dogs. Dig Dis Sci. 1988; 33(7):828-32. DOI: 10.1007/BF01550971. View

Lundberg J, Terenius L, Hokfelt T, Martling C, Tatemoto K, Mutt V . Neuropeptide Y (NPY)-like immunoreactivity in peripheral noradrenergic neurons and effects of NPY on sympathetic function. Acta Physiol Scand. 1982; 116(4):477-80. DOI: 10.1111/j.1748-1716.1982.tb07171.x. View

Allen J, Bircham P, Bloom S, Edwards A . Release of neuropeptide Y in response to splanchnic nerve stimulation in the conscious calf. J Physiol. 1984; 357:401-8. PMC: 1193265. DOI: 10.1113/jphysiol.1984.sp015507. View

Lundberg J, Tatemoto K . Pancreatic polypeptide family (APP, BPP, NPY and PYY) in relation to sympathetic vasoconstriction resistant to alpha-adrenoceptor blockade. Acta Physiol Scand. 1982; 116(4):393-402. DOI: 10.1111/j.1748-1716.1982.tb07157.x. View

10.

Dahlof C, Dahlof P, Lundberg J . Neuropeptide Y (NPY): enhancement of blood pressure increase upon alpha-adrenoceptor activation and direct pressor effects in pithed rats. Eur J Pharmacol. 1985; 109(2):289-92. DOI: 10.1016/0014-2999(85)90433-9. View

11.

Inoue K, Kawano T, Shima K, Kim T, Suzuki T, Tobe T . Effect of synthetic chicken vasoactive intestinal peptide on pancreatic blood flow and on exocrine and endocrine secretions of the pancreas in dogs. Dig Dis Sci. 1983; 28(8):724-32. DOI: 10.1007/BF01312563. View

12.

von Kugelgen I, Starke K . Noradrenaline and adenosine triphosphate as co-transmitters of neurogenic vasoconstriction in rabbit mesenteric artery. J Physiol. 1985; 367:435-55. PMC: 1193073. DOI: 10.1113/jphysiol.1985.sp015834. View

13.

Beijer H, Maas A, Charbon G . A vasopressin-induced decrease in pancreatic blood flow and in pancreatic exocrine secretion in the anesthetized dog. Pflugers Arch. 1984; 400(3):324-8. DOI: 10.1007/BF00581567. View

14.

Kiyama S, Ooi Y, Kitagawa K, Nakamura T, Akita T, Kogire M . Synthesis of porcine neuropeptide Y(NPY) in solution. Int J Pept Protein Res. 1987; 29(4):533-44. DOI: 10.1111/j.1399-3011.1987.tb02281.x. View

15.

Doi R, Inoue K, Kogire M, Sumi S, Takaori K, Suzuki T . Simultaneous measurement of hepatic arterial and portal venous flows by transit time ultrasonic volume flowmetry. Surg Gynecol Obstet. 1988; 167(1):65-9. View

16.

Konturek S, Bilski J, Pawlik W, TASLER J, Domschke W . Adrenergic pathway in the inhibition of pancreatic secretion by peptide YY in dogs. Gastroenterology. 1988; 94(2):266-73. DOI: 10.1016/0016-5085(88)90412-x. View

17.

Carlei F, Allen J, Bishop A, Bloom S, Polak J . Occurrence, distribution and nature of neuropeptide Y in the rat pancreas. Experientia. 1985; 41(12):1554-7. DOI: 10.1007/BF01964804. View

18.

GOODHEAD B, Himal H, Zanbilowicz J . Relationship between pancreatic secretion and pancreatic blood flow. Gut. 1970; 11(1):62-8. PMC: 1411372. DOI: 10.1136/gut.11.1.62. View

19.

Kvietys P, McLendon J, Bulkley G, Perry M, Granger D . Pancreatic circulation: intrinsic regulation. Am J Physiol. 1982; 242(6):G596-602. DOI: 10.1152/ajpgi.1982.242.6.G596. View

20.

Lundberg J, Anggard A, Pernow J, Hokfelt T . Neuropeptide Y-, substance P- and VIP-immunoreactive nerves in cat spleen in relation to autonomic vascular and volume control. Cell Tissue Res. 1985; 239(1):9-18. DOI: 10.1007/BF00214896. View