A Pyrrolysine Analogue for Protein Click Chemistry

Overview

Journal Angew Chem Int Ed Engl

Specialty Chemistry

Date 2009 Jan 22

PMID 19156778

Citations 36

Authors

Tomasz Fekner

Xin Li

Marianne M Lee

Michael K Chan

Affiliations

Soon will be listed here.

Abstract

Ignoring the STOP sign: A pyrrolysine analogue bearing a terminal alkyne was site-specifically incorporated into recombinant calmodulin (CaM) through a UAG codon. The resulting protein was labeled with an azide-containing dye using a copper(I)-catalyzed click reaction. Subsequent application of an orthogonal cysteine tagging method yielded a CaM labeled with two distinct fluorophores that enabled its study by FRET spectroscopy.

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