In-depth Proteomics to Define the Cell Surface and Secretome of Ovarian Cancer Cells and Processes of Protein Shedding

Overview

Journal Cancer Res

Specialty Oncology

Date 2009 Jan 22

PMID 19155298

Citations 24

Authors

Vitor M Faca

Samir M Hanash

Affiliations

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Abstract

Current proteomics technologies allow substantial depth of analysis of cellular and subcellular proteomes as shown in the proteomic profiling of ovarian cancer cells. This in-depth analysis has elucidated the repertoire of proteins expressed on the cell surface and proteins released into the extracellular milieu, uncovering extensive shedding of extracellular domains of cell adhesion proteins and a highly dynamic protein secretion process. The protein sets identified provide a rich resource of potential circulating markers and targets for imaging and therapeutics for ovarian cancer.

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References

de Wever O, Derycke L, Hendrix A, De Meerleer G, Godeau F, Depypere H . Soluble cadherins as cancer biomarkers. Clin Exp Metastasis. 2007; 24(8):685-97. DOI: 10.1007/s10585-007-9104-8. View

Sodek K, Evangelou A, Ignatchenko A, Agochiya M, Brown T, Ringuette M . Identification of pathways associated with invasive behavior by ovarian cancer cells using multidimensional protein identification technology (MudPIT). Mol Biosyst. 2008; 4(7):762-73. DOI: 10.1039/b717542f. View

Gortzak-Uzan L, Ignatchenko A, Evangelou A, Agochiya M, Brown K, St Onge P . A proteome resource of ovarian cancer ascites: integrated proteomic and bioinformatic analyses to identify putative biomarkers. J Proteome Res. 2007; 7(1):339-51. DOI: 10.1021/pr0703223. View

Nyormoi O, Bar-Eli M . Transcriptional regulation of metastasis-related genes in human melanoma. Clin Exp Metastasis. 2003; 20(3):251-63. DOI: 10.1023/a:1022991302172. View

Lutz A, Willmann J, Cochran F, Ray P, Gambhir S . Cancer screening: a mathematical model relating secreted blood biomarker levels to tumor sizes. PLoS Med. 2008; 5(8):e170. PMC: 2517618. DOI: 10.1371/journal.pmed.0050170. View

Hamilton T, Young R, McKoy W, Grotzinger K, Green J, Chu E . Characterization of a human ovarian carcinoma cell line (NIH:OVCAR-3) with androgen and estrogen receptors. Cancer Res. 1983; 43(11):5379-89. View

Wu G, Fu P, Chiang C, Huss W, Greenberg N, Wu M . Increased expression of MUC18 correlates with the metastatic progression of mouse prostate adenocarcinoma in the TRAMP model. J Urol. 2005; 173(5):1778-83. DOI: 10.1097/01.ju.0000154643.30048.2c. View

Scholler N, Fu N, Yang Y, Ye Z, Goodman G, Hellstrom K . Soluble member(s) of the mesothelin/megakaryocyte potentiating factor family are detectable in sera from patients with ovarian carcinoma. Proc Natl Acad Sci U S A. 1999; 96(20):11531-6. PMC: 18068. DOI: 10.1073/pnas.96.20.11531. View

Kislinger T, Cox B, Kannan A, Chung C, Hu P, Ignatchenko A . Global survey of organ and organelle protein expression in mouse: combined proteomic and transcriptomic profiling. Cell. 2006; 125(1):173-86. DOI: 10.1016/j.cell.2006.01.044. View

10.

Kulasingam V, Diamandis E . Strategies for discovering novel cancer biomarkers through utilization of emerging technologies. Nat Clin Pract Oncol. 2008; 5(10):588-99. DOI: 10.1038/ncponc1187. View

11.

Hanash S, Pitteri S, Faca V . Mining the plasma proteome for cancer biomarkers. Nature. 2008; 452(7187):571-9. DOI: 10.1038/nature06916. View

12.

Sawada K, Mitra A, Radjabi A, Bhaskar V, Kistner E, Tretiakova M . Loss of E-cadherin promotes ovarian cancer metastasis via alpha 5-integrin, which is a therapeutic target. Cancer Res. 2008; 68(7):2329-39. PMC: 2665934. DOI: 10.1158/0008-5472.CAN-07-5167. View

13.

Karlan B, Jones J, Slamon D, Lagasse L . Glucocorticoids stabilize HER-2/neu messenger RNA in human epithelial ovarian carcinoma cells. Gynecol Oncol. 1994; 53(1):70-7. DOI: 10.1006/gyno.1994.1090. View

14.

Faca V, Ventura A, Fitzgibbon M, Pereira-Faca S, Pitteri S, Green A . Proteomic analysis of ovarian cancer cells reveals dynamic processes of protein secretion and shedding of extra-cellular domains. PLoS One. 2008; 3(6):e2425. PMC: 2409963. DOI: 10.1371/journal.pone.0002425. View

15.

Liu H, Sadygov R, Yates 3rd J . A model for random sampling and estimation of relative protein abundance in shotgun proteomics. Anal Chem. 2004; 76(14):4193-201. DOI: 10.1021/ac0498563. View

16.

Rosso O, Piazza T, Bongarzone I, Rossello A, Mezzanzanica D, Canevari S . The ALCAM shedding by the metalloprotease ADAM17/TACE is involved in motility of ovarian carcinoma cells. Mol Cancer Res. 2008; 5(12):1246-53. DOI: 10.1158/1541-7786.MCR-07-0060. View

17.

Ahmed N, Thompson E, Quinn M . Epithelial-mesenchymal interconversions in normal ovarian surface epithelium and ovarian carcinomas: an exception to the norm. J Cell Physiol. 2007; 213(3):581-8. DOI: 10.1002/jcp.21240. View

18.

Symowicz J, Adley B, Gleason K, Johnson J, Ghosh S, Fishman D . Engagement of collagen-binding integrins promotes matrix metalloproteinase-9-dependent E-cadherin ectodomain shedding in ovarian carcinoma cells. Cancer Res. 2007; 67(5):2030-9. DOI: 10.1158/0008-5472.CAN-06-2808. View

19.

Jemal A, Siegel R, Ward E, Murray T, Xu J, Thun M . Cancer statistics, 2007. CA Cancer J Clin. 2007; 57(1):43-66. DOI: 10.3322/canjclin.57.1.43. View

20.

Naora H, Montell D . Ovarian cancer metastasis: integrating insights from disparate model organisms. Nat Rev Cancer. 2005; 5(5):355-66. DOI: 10.1038/nrc1611. View