Dissecting the Splicing Mechanism of the Drosophila Editing Enzyme; DADAR

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2009 Jan 21

PMID 19153139

Citations 1

Authors

Roberto Marcucci

Maurizio Romano

Fabian Feiguin

Mary A OConnell

Francisco E Baralle

Affiliations

Soon will be listed here.

Abstract

In Drosophila melanogaster, the expression of adenosine deaminase acting on RNA is regulated by transcription and alternative splicing so that at least four different isoforms are generated that have a tissue-specific splicing pattern. Even though dAdar has been extensively studied, the complete adult expression pattern has yet to be elucidated. In the present study, we investigate mature transcripts of dAdar arising from different promoters. Two predominant isoforms of dAdar are expressed in gonads and dAdar is transcribed from both the embryonic and the adult promoters. Furthermore, full-length transcripts containing the alternatively spliced exon-1 are expressed in a tissue-specific manner. The splicing factor B52/SRp55 binds within the alternative spliced exon 3a and plays a role in this alternative splicing event.

Citing Articles

Engineered alterations in RNA editing modulate complex behavior in Drosophila: regulatory diversity of adenosine deaminase acting on RNA (ADAR) targets.

Jepson J, Savva Y, Yokose C, Sugden A, Sahin A, Reenan R J Biol Chem. 2010; 286(10):8325-8337.

PMID: 21078670 PMC: 3048717. DOI: 10.1074/jbc.M110.186817.

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