Control of Yeast GAL Genes by MIG1 Repressor: a Transcriptional Cascade in the Glucose Response

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1991 Nov 1

PMID 1915298

Citations 169

Authors

J O Nehlin

M Carlberg

H Ronne

Affiliations

Soon will be listed here.

Abstract

Glucose repression is a global regulatory mechanism in yeast. We have investigated how glucose regulates the GAL genes, which are required for galactose fermentation. We found that the GAL genes are controlled by a transcriptional cascade. Thus, GAL4, which encodes an activator of the GAL genes, is repressed by MIG1, a zinc finger protein that binds to the GAL4 promoter. MIG1 has a dual role in control of the GAL genes, since MIG1 also binds to the promoter of GAL1, a gene regulated by GAL4. A disruption of MIG1 interacts synergistically with a disruption of GAL80, a gene involved in galactose induction. This suggests that the MIG1-dependent response to glucose is amplified by down-regulation of the induction pathway.

Citing Articles

A highly selective cell-based fluorescent biosensor for genistein detection.

Chao L, Liu D, Siewers V Eng Microbiol. 2024; 3(2):100078.

PMID: 39629249 PMC: 11611022. DOI: 10.1016/j.engmic.2023.100078.

A unique metabolic gene cluster regulates lactose and galactose metabolism in the yeast .

Peri K, Yuan L, Faria Oliveira F, Persson K, Alalam H, Olsson L Appl Environ Microbiol. 2024; 90(10):e0113524.

PMID: 39240082 PMC: 11497787. DOI: 10.1128/aem.01135-24.

LowTempGAL: a highly responsive low temperature-inducible GAL system in Saccharomyces cerevisiae.

Lu Z, Shen Q, Bandari N, Evans S, McDonnell L, Liu L Nucleic Acids Res. 2024; 52(12):7367-7383.

PMID: 38808673 PMC: 11229376. DOI: 10.1093/nar/gkae460.

Natural promoters and promoter engineering strategies for metabolic regulation in Saccharomyces cerevisiae.

He S, Zhang Z, Lu W J Ind Microbiol Biotechnol. 2023; 50(1).

PMID: 36633543 PMC: 9936215. DOI: 10.1093/jimb/kuac029.

A new platform host for strong expression under promoters without inducer in .

Kim M, Sung B, Park H, Sohn J, Bae J Biotechnol Rep (Amst). 2022; 36:e00763.

PMID: 36159742 PMC: 9493058. DOI: 10.1016/j.btre.2022.e00763.

References

DOUGLAS H, Hawthorne D . ENZYMATIC EXPRESSION AND GENETIC LINKAGE OF GENES CONTROLLING GALACTOSE UTILIZATION IN SACCHAROMYCES. Genetics. 1964; 49:837-44. PMC: 1210618. DOI: 10.1093/genetics/49.5.837. View

Entian K . Glucose repression: a complex regulatory system in yeast. Microbiol Sci. 1986; 3(12):366-71. View

Finley Jr R, Chen S, Ma J, Byrne P, West Jr R . Opposing regulatory functions of positive and negative elements in UASG control transcription of the yeast GAL genes. Mol Cell Biol. 1990; 10(11):5663-70. PMC: 361331. DOI: 10.1128/mcb.10.11.5663-5670.1990. View

Johnston M . A model fungal gene regulatory mechanism: the GAL genes of Saccharomyces cerevisiae. Microbiol Rev. 1987; 51(4):458-76. PMC: 373127. DOI: 10.1128/mr.51.4.458-476.1987. View

Giniger E, Ptashne M . Cooperative DNA binding of the yeast transcriptional activator GAL4. Proc Natl Acad Sci U S A. 1988; 85(2):382-6. PMC: 279552. DOI: 10.1073/pnas.85.2.382. View

Tschopp J, Emr S, Field C, Schekman R . GAL2 codes for a membrane-bound subunit of the galactose permease in Saccharomyces cerevisiae. J Bacteriol. 1986; 166(1):313-8. PMC: 214594. DOI: 10.1128/jb.166.1.313-318.1986. View

Bajwa W, Torchia T, Hopper J . Yeast regulatory gene GAL3: carbon regulation; UASGal elements in common with GAL1, GAL2, GAL7, GAL10, GAL80, and MEL1; encoded protein strikingly similar to yeast and Escherichia coli galactokinases. Mol Cell Biol. 1988; 8(8):3439-47. PMC: 363581. DOI: 10.1128/mcb.8.8.3439-3447.1988. View

Ptashne M . How eukaryotic transcriptional activators work. Nature. 1988; 335(6192):683-9. DOI: 10.1038/335683a0. View

Nehlin J, Carlberg M, Ronne H . Yeast galactose permease is related to yeast and mammalian glucose transporters. Gene. 1989; 85(2):313-9. DOI: 10.1016/0378-1119(89)90423-x. View

10.

Thomas B, Rothstein R . Elevated recombination rates in transcriptionally active DNA. Cell. 1989; 56(4):619-30. DOI: 10.1016/0092-8674(89)90584-9. View

11.

Torchia T, Hamilton R, Cano C, Hopper J . Disruption of regulatory gene GAL80 in Saccharomyces cerevisiae: effects on carbon-controlled regulation of the galactose/melibiose pathway genes. Mol Cell Biol. 1984; 4(8):1521-7. PMC: 368943. DOI: 10.1128/mcb.4.8.1521-1527.1984. View

12.

Johnston M, Davis R . Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae. Mol Cell Biol. 1984; 4(8):1440-8. PMC: 368932. DOI: 10.1128/mcb.4.8.1440-1448.1984. View

13.

Yocum R, Hanley S, West Jr R, Ptashne M . Use of lacZ fusions to delimit regulatory elements of the inducible divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae. Mol Cell Biol. 1984; 4(10):1985-98. PMC: 369015. DOI: 10.1128/mcb.4.10.1985-1998.1984. View

14.

Nogi Y, Fukasawa T . Nucleotide sequence of the yeast regulatory gene GAL80. Nucleic Acids Res. 1984; 12(24):9287-98. PMC: 320461. DOI: 10.1093/nar/12.24.9287. View

15.

Carlson M . Regulation of sugar utilization in Saccharomyces species. J Bacteriol. 1987; 169(11):4873-7. PMC: 213879. DOI: 10.1128/jb.169.11.4873-4877.1987. View

16.

Matsumoto K, Toh-E A, Oshima Y . Isolation and characterization of dominant mutations resistant to carbon catabolite repression of galactokinase synthesis in Saccharomyces cerevisiae. Mol Cell Biol. 1981; 1(2):83-93. PMC: 369646. DOI: 10.1128/mcb.1.2.83-93.1981. View

17.

Nehlin J, Ronne H . Yeast MIG1 repressor is related to the mammalian early growth response and Wilms' tumour finger proteins. EMBO J. 1990; 9(9):2891-8. PMC: 552004. DOI: 10.1002/j.1460-2075.1990.tb07479.x. View

18.

Flick J, Johnston M . Two systems of glucose repression of the GAL1 promoter in Saccharomyces cerevisiae. Mol Cell Biol. 1990; 10(9):4757-69. PMC: 361077. DOI: 10.1128/mcb.10.9.4757-4769.1990. View

19.

Mylin L, Johnston M, Hopper J . Phosphorylated forms of GAL4 are correlated with ability to activate transcription. Mol Cell Biol. 1990; 10(9):4623-9. PMC: 361051. DOI: 10.1128/mcb.10.9.4623-4629.1990. View

20.

Johnston S, Hopper J . Isolation of the yeast regulatory gene GAL4 and analysis of its dosage effects on the galactose/melibiose regulon. Proc Natl Acad Sci U S A. 1982; 79(22):6971-5. PMC: 347256. DOI: 10.1073/pnas.79.22.6971. View