Production of Nisin Z Using Lactococcus Lactis IO-1 from Hydrolyzed Sago Starch

Overview

Journal J Ind Microbiol Biotechnol

Publisher Oxford University Press

Specialty Biotechnology

Date 2009 Jan 13

PMID 19137340

Citations 3

Authors

Octavio Carvajal-Zarrabal

Cirilo Nolasco-Hipolito

Kopli B Bujang

Ayaaki Ishizaki

Affiliations

Soon will be listed here.

Abstract

A membrane bioreactor for production of nisin Z was constructed using Lactococcus lactis IO-1 in continuous culture using hydrolyzed sago starch as carbon source. A strategy used to enhance the productivity of nisin Z was to maintain the cells in a continuous growth at high cell concentration. This resulted in a volumetric productivity of nisin Z, as 50,000 IU l(-1) h(-1) using a cell concentration of 15 g l(-1), 30( degrees )C, pH 5.5 and a dilution rate of 1.24 h(-1). Adding 10 g l(-1) YE and 2 g l(-1) polypeptone, other inducers were unnecessary to maintain production of nisin. The operating conditions of the reactor removed nisin and lactate, thus minimizing their effects which allowed the maintenance of cells in continuous exponential growth phase mode with high metabolic activity.

Citing Articles

Improving nitrogen source utilization from defatted soybean meal for nisin production by enhancing proteolytic function of Lactococcus lactis F44.

Liu J, Zhou J, Wang L, Ma Z, Zhao G, Ge Z Sci Rep. 2017; 7(1):6189.

PMID: 28733629 PMC: 5522456. DOI: 10.1038/s41598-017-06537-w.

Lactic acid production by Enteroccocus faecium in liquefied sago starch.

Nolasco-Hipolito C, Zarrabal O, Kamaldin R, Teck-Yee L, Lihan S, Bujang K AMB Express. 2012; 2(1):53.

PMID: 23021076 PMC: 3492075. DOI: 10.1186/2191-0855-2-53.

Continuous nisin production with bioengineered Lactococcus lactis strains.

Simsek O, Akkoc N, Con A, Ozcelik F, Saris P, Akcelik M J Ind Microbiol Biotechnol. 2009; 36(6):863-71.

PMID: 19337764 DOI: 10.1007/s10295-009-0563-6.

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