Chitin Membrane for Wound Dressing Application--preparation, Characterisation and Toxicological Evaluation

Overview

Journal Int Wound J

Specialties Emergency Medicine
Orthopedics

Date 2009 Jan 13

PMID 19134068

Citations 7

Authors

Rita Singh

Madhukar Parashuram Chacharkar

Adesh Kumar Mathur

Affiliations

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Abstract

Chitin, a unique biopolymer based on the N-acetyl-glucosamine monomer is envisioned to promote rapid dermal regeneration and accelerate wound healing. It has many useful and advantageous biological properties for its application as a wound dressing. Chitin membranes were prepared using lithium chloride/dimethylacetamide solvent system and evaluated for use as a wound dressing. Swelling behaviour, moisture vapour transmission rate, microbial impermeability and antimicrobial efficacy of the dressings was evaluated. The chitin dressing provided an effective barrier to microbial penetration and exerted a broad bacteriostatic action against Gram-positive and Gram-negative organisms. Gamma irradiation at 25 kGy was found suitable for sterilisation of the dressings. The thermal decomposition of unirradiated and irradiated chitin membranes was investigated. No significant change in the thermal behaviour because of irradiation at 25 kGy was observed. In vitro biodegradation of unirradiated and irradiated chitin membranes showed the susceptibility of the chitin dressing to lysozyme. Irritant effect of the chitin membrane dressings on skin was tested. Subcutaneous and scarification test in guinea pigs showed no signs of inflammation. This was further supported by the Finkelstein's test performed in rabbits. The chitin membranes were found to have optimal performance characteristics of a wound dressing and showed no toxicity or possible adverse reactions. The study shows the chitin dressings as useful adjunct in wound care.

Citing Articles

Extraction and Physicochemical Characterization of Chitin from Sloughs of the South-Eastern French Mediterranean Basin.

Poerio A, Petit C, Jehl J, Arab-Tehrany E, Mano J, Cleymand F Molecules. 2020; 25(11).

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Chitin and chitosan: biopolymers for wound management.

Singh R, Shitiz K, Singh A Int Wound J. 2017; 14(6):1276-1289.

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Fabrication, Characterization, and Evaluation of Bionanocomposites Based on Natural Polymers and Antibiotics for Wound Healing Applications.

Radulescu M, Holban A, Mogoanta L, Balseanu T, Mogosanu G, Savu D Molecules. 2016; 21(6).

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Evaluation of Antimicrobial and Healing Activities of Frog Skin on Guinea Pigs Wounds.

Rezazade Bazaz M, Mashreghi M, Shahri N, Mashreghi M, Asoodeh A, Rassouli M Jundishapur J Microbiol. 2015; 8(8):e21218.

PMID: 26468364 PMC: 4600859. DOI: 10.5812/jjm.21218v2.

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