A Microfluidic Device for Multiplexed Protein Detection in Nano-liter Volumes

Overview

Journal Anal Biochem

Publisher Elsevier

Specialty Biochemistry

Date 2009 Jan 10

PMID 19133224

Citations 34

Authors

Alan H Diercks

Adrian Ozinsky

Carl L Hansen

James M Spotts

David J Rodriguez

Alan Aderem

Affiliations

Soon will be listed here.

Abstract

We describe a microfluidic immunoassay device that permits sensitive and quantitative multiplexed protein measurements on nano-liter-scale samples. The device exploits the combined power of integrated microfluidics and optically encoded microspheres to create an array of approximately 100-microm(2) sensors functionalized with capture antibodies directed against distinct targets. This strategy overcomes the need for performing biochemical coupling of affinity reagents to the device substrate, permits multiple proteins to be detected in a nano-liter-scale sample, is scalable to large numbers of samples, and has the required sensitivity to measure the abundance of proteins derived from single mammalian cells. The sensitivity of the device is sufficient to detect 1000 copies of tumor necrosis factor (TNF) in a volume of 4.7nl.

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