Dictyostelium Discoideum: a Model Host to Measure Bacterial Virulence

Overview

Journal Nat Protoc

Specialties Biology
Pathology
Science

Date 2009 Jan 10

PMID 19131953

Citations 51

Authors

Romain Froquet

Emmanuelle Lelong

Anna Marchetti

Pierre Cosson

Affiliations

Soon will be listed here.

Abstract

Dictyostelium amoebae have been used as a host model to measure virulence of a wide range of bacterial pathogens. The simple protocol described here takes advantage of the ability of Dictyostelium to grow and form plaques on a lawn of nonpathogenic bacteria but not on virulent bacteria. This assay can be modulated to measure the virulence of different bacterial pathogens. By adjusting various parameters such as cell numbers or media, a more quantitative measure of bacterial virulence can also be obtained. The entire procedure takes about 5 h to compete, and up to 2 further weeks may be required for plaques to form on the bacterial lawn.

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