Cotranscriptional Recruitment of the MRNA Export Factor Yra1 by Direct Interaction with the 3' End Processing Factor Pcf11

Overview

Journal Mol Cell

Publisher Cell Press

Specialty Cell Biology

Date 2008 Dec 27

PMID 19110458

Citations 90

Authors

Sara Ann Johnson

Gabrielle Cubberley

David L Bentley

Affiliations

Soon will be listed here.

Abstract

We investigated recruitment of the yeast mRNA export factor Yra1 to the transcription elongation complex (TEC). Previously, the Sub2 helicase subunit of TREX was proposed to recruit Yra1. We report that Sub2 is dispensable for Yra1 recruitment, but the cleavage/polyadenylation factor, CF1A, is required. Yra1 binds directly to the Zn finger/Clp1 region of Pcf11, the pol II CTD-binding subunit of CF1A, and this interaction is conserved between their human homologs. Tethering of Pcf11 to nascent mRNA is sufficient to enhance Yra1 recruitment. Interaction with Pcf11 can therefore explain Yra1 binding to the TEC independently of Sub2. We propose that after initially binding to Pcf11, Yra1 is transferred to Sub2. Consistent with this idea, Pcf11 binds the same regions of Yra1 that also contact Sub2, indicating a mutually exclusive interaction. These results suggest a mechanism for cotranscriptional assembly of the export competent mRNP and for coordinating export with 3' end processing.

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