A Novel Chair-type G-quadruplex Formed by a Bombyx Mori Telomeric Sequence

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2008 Dec 24

PMID 19103662

Citations 20

Authors

Samir Amrane

Rita Wan Lin Ang

Zhong Ming Tan

Chun Li

Joefina Kim Cheow Lim

Jocelyn Mei Wen Lim

Kah Wai Lim

Anh Tuan Phan

Affiliations

Soon will be listed here.

Abstract

Recently, the human telomeric d[TAGGG(TTAGGG)(3)] sequence has been shown to form in K(+) solution an intramolecular (3+1) G-quadruplex structure, whose G-tetrad core contains three strands oriented in one direction and the fourth in the opposite direction. Here we present a study on the structure of the Bombyx mori telomeric d[TAGG(TTAGG)(3)] sequence, which differs from the human counterpart only by one G deletion in each repeat. We found that this sequence adopted multiple G-quadruplex structures in K(+) solution. We have favored a major G-quadruplex form by a judicious U-for-T substitution in the sequence and determined the folding topology of this form. We showed by NMR that this was a new chair-type intramolecular G-quadruplex which involved a two-layer antiparallel G-tetrad core and three edgewise loops. Our result highlights the effect of G-tract length on the folding topology of G-quadruplexes, but also poses the question of whether a similar chair-type G-quadruplex fold exists in the human telomeric sequences.

Citing Articles

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In vivo dynamics and regulation of DNA G-quadruplex structures in mammals.

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