Comparative Study of Three Analysis Methods (TTGE, Flow Cytometry and HPLC) for Xenobiotic Impact Assessment on Phytoplankton Communities

Overview

Journal Ecotoxicology

Specialties Environmental Health
Toxicology

Date 2008 Dec 20

PMID 19096934

Citations 1

Authors

Sabine Stachowski-Haberkorn

Louis Quiniou

Beatriz Beker

Hansy Haberkorn

Dominique Marie

Denis de la Broise

Affiliations

Soon will be listed here.

Abstract

The impacts of the fungicide Opus (epoxiconazole) on marine phytoplankton communities were assessed in a 12-day field experiment using in situ microcosms maintained underwater at 6 m depth. Three community analysis methods were compared for their sensitivity threshold in fungicide impact detection. When phytoplankton communities were exposed to 1 microg l(-1) of epoxiconazole, no effects could be demonstrated using TTGE (Temporal Temperature Gradient gel Electrophoresis), flow cytometry or HPLC. When exposed to 10 microg l(-1), TTGE fingerprints from PCR amplified 18S rDNA of communities exhibited significant differences compared with controls (ANOSIM, P = 0.028). Neither flow cytometry counts, nor HPLC pigment profiles allowed to show significant differences in microcosms exposed to 10 microg l(-1) of epoxiconazole. When exposed to 100 microg l(-1), all three methods allowed to detect significant differences in treated microcosms, as compared to controls. The TTGE analysis appears in this study as the most sensitive method for fungicide impact assessment on eukaryote microbial communities.

Citing Articles

Phytoplankton distribution patterns in the northwestern Sargasso Sea revealed by small subunit rRNA genes from plastids.

Treusch A, Demir-Hilton E, Vergin K, Worden A, Carlson C, Donatz M ISME J. 2011; 6(3):481-92.

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