A Nitrile Hydratase in the Eukaryote Monosiga Brevicollis

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2008 Dec 20

PMID 19096720

Citations 13

Authors

Konrad U Foerstner

Tobias Doerks

Jean Muller

Jeroen Raes

Peer Bork

Affiliations

Soon will be listed here.

Abstract

Bacterial nitrile hydratase (NHases) are important industrial catalysts and waste water remediation tools. In a global computational screening of conventional and metagenomic sequence data for NHases, we detected the two usually separated NHase subunits fused in one protein of the choanoflagellate Monosiga brevicollis, a recently sequenced unicellular model organism from the closest sister group of Metazoa. This is the first time that an NHase is found in eukaryotes and the first time it is observed as a fusion protein. The presence of an intron, subunit fusion and expressed sequence tags covering parts of the gene exclude contamination and suggest a functional gene. Phylogenetic analyses and genomic context imply a probable ancient horizontal gene transfer (HGT) from proteobacteria. The newly discovered NHase might open biotechnological routes due to its unconventional structure, its new type of host and its apparent integration into eukaryotic protein networks.

Citing Articles

Nitrile hydratase as a promising biocatalyst: recent advances and future prospects.

Feng C, Chen J, Ye W, Wang Z Biotechnol Lett. 2024; 46(6):1171-1185.

PMID: 39269672 DOI: 10.1007/s10529-024-03530-y.

Recent Advances and Promises in Nitrile Hydratase: From Mechanism to Industrial Applications.

Cheng Z, Xia Y, Zhou Z Front Bioeng Biotechnol. 2020; 8:352.

PMID: 32391348 PMC: 7193024. DOI: 10.3389/fbioe.2020.00352.

Advances in cloning, structural and bioremediation aspects of nitrile hydratases.

Supreetha K, Rao S, Srividya D, Anil H, Kiran S Mol Biol Rep. 2019; 46(4):4661-4673.

PMID: 31201677 DOI: 10.1007/s11033-019-04811-w.

Teneurin Structures Are Composed of Ancient Bacterial Protein Domains.

Jackson V, Busby J, Janssen B, Lott J, Seiradake E Front Neurosci. 2019; 13:183.

PMID: 30930731 PMC: 6425310. DOI: 10.3389/fnins.2019.00183.

Analyzing the function of the insert region found between the α and β-subunits in the eukaryotic nitrile hydratase from Monosiga brevicollis.

Yang X, Bennett B, Holz R Arch Biochem Biophys. 2018; 657:1-7.

PMID: 30205086 PMC: 6201762. DOI: 10.1016/j.abb.2018.08.013.

References

Digeronimo M, Antoine A . Metabolism of acetonitrile and propionitrile by Nocardia rhodochrous LL100-21. Appl Environ Microbiol. 1976; 31(6):900-6. PMC: 169854. DOI: 10.1128/aem.31.6.900-906.1976. View

Langdahl B, Bisp P, Ingvorsen K . Nitrile hydrolysis by BL1, an acetonitrile-tolerant strain isolated from a marine sediment. Microbiology (Reading). 2021; 142(1):145-154. DOI: 10.1099/13500872-142-1-145. View

LARKIN M, Blackshields G, Brown N, Chenna R, McGettigan P, McWilliam H . Clustal W and Clustal X version 2.0. Bioinformatics. 2007; 23(21):2947-8. DOI: 10.1093/bioinformatics/btm404. View

Tyson G, Chapman J, Hugenholtz P, Allen E, Ram R, Richardson P . Community structure and metabolism through reconstruction of microbial genomes from the environment. Nature. 2004; 428(6978):37-43. DOI: 10.1038/nature02340. View

Layh N, Stolz A, Bohme J, Effenberger F, Knackmuss H . Enantioselective hydrolysis of racemic naproxen nitrile and naproxen amide to S-naproxen by new bacterial isolates. J Biotechnol. 1994; 33(2):175-82. DOI: 10.1016/0168-1656(94)90109-0. View

Pereira R, Graham D, Rainey F, Cowan D . A novel thermostable nitrile hydratase. Extremophiles. 1998; 2(3):347-57. DOI: 10.1007/s007920050078. View

DeLong E, Preston C, Mincer T, Rich V, Hallam S, Frigaard N . Community genomics among stratified microbial assemblages in the ocean's interior. Science. 2006; 311(5760):496-503. DOI: 10.1126/science.1120250. View

Banerjee A, Sharma R, Banerjee U . The nitrile-degrading enzymes: current status and future prospects. Appl Microbiol Biotechnol. 2002; 60(1-2):33-44. DOI: 10.1007/s00253-002-1062-0. View

Tringe S, von Mering C, Kobayashi A, Salamov A, Chen K, Chang H . Comparative metagenomics of microbial communities. Science. 2005; 308(5721):554-7. DOI: 10.1126/science.1107851. View

10.

Eddy S . Profile hidden Markov models. Bioinformatics. 1999; 14(9):755-63. DOI: 10.1093/bioinformatics/14.9.755. View

11.

Mitra S, Holz R . Unraveling the catalytic mechanism of nitrile hydratases. J Biol Chem. 2006; 282(10):7397-404. DOI: 10.1074/jbc.M604117200. View

12.

Huang W, Jia J, Cummings J, Nelson M, Schneider G, Lindqvist Y . Crystal structure of nitrile hydratase reveals a novel iron centre in a novel fold. Structure. 1997; 5(5):691-9. DOI: 10.1016/s0969-2126(97)00223-2. View

13.

Rutherford K, Parkhill J, Crook J, Horsnell T, Rice P, Rajandream M . Artemis: sequence visualization and annotation. Bioinformatics. 2000; 16(10):944-5. DOI: 10.1093/bioinformatics/16.10.944. View

14.

Edgar R . MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 2004; 32(5):1792-7. PMC: 390337. DOI: 10.1093/nar/gkh340. View

15.

Suzek B, Huang H, McGarvey P, Mazumder R, Wu C . UniRef: comprehensive and non-redundant UniProt reference clusters. Bioinformatics. 2007; 23(10):1282-8. DOI: 10.1093/bioinformatics/btm098. View

16.

Brandao P, Bull A . Nitrile hydrolysing activities of deep-sea and terrestrial mycolate actinomycetes. Antonie Van Leeuwenhoek. 2003; 84(2):89-98. DOI: 10.1023/a:1025409818275. View

17.

Rusch D, Halpern A, Sutton G, Heidelberg K, Williamson S, Yooseph S . The Sorcerer II Global Ocean Sampling expedition: northwest Atlantic through eastern tropical Pacific. PLoS Biol. 2007; 5(3):e77. PMC: 1821060. DOI: 10.1371/journal.pbio.0050077. View

18.

Nakasako M, Odaka M, Yohda M, Dohmae N, Takio K, Kamiya N . Tertiary and quaternary structures of photoreactive Fe-type nitrile hydratase from Rhodococcus sp. N-771: roles of hydration water molecules in stabilizing the structures and the structural origin of the substrate specificity of the enzyme. Biochemistry. 1999; 38(31):9887-98. DOI: 10.1021/bi982753s. View

19.

Letunic I, Bork P . Interactive Tree Of Life (iTOL): an online tool for phylogenetic tree display and annotation. Bioinformatics. 2006; 23(1):127-8. DOI: 10.1093/bioinformatics/btl529. View

20.

Colquhoun J, Heald S, Li L, Tamaoka J, Kato C, Horikoshi K . Taxonomy and biotransformation activities of some deep-sea actinomycetes. Extremophiles. 1998; 2(3):269-77. DOI: 10.1007/s007920050069. View