Human Neutrophils Switch to an Activated Phenotype After Homing to the Lung Irrespective of Inflammatory Disease

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 2008 Dec 17

PMID 19077082

Citations 94

Authors

E Fortunati

K M Kazemier

J C Grutters

L Koenderman

van J M M Van den Bosch

Affiliations

Soon will be listed here.

Abstract

Systemic inflammation can be investigated by changes in expression profiles of neutrophil receptors. Application of this technology for analysis of neutrophil phenotypes in diseased tissues is hampered by the absence of information regarding the modulation of neutrophil phenotypes after extravasation to tissues under non-inflammatory conditions. To fill this gap we measured the expression of neutrophil receptors in bronchoalveolar lavage fluid (BALF) and in the peripheral blood of healthy volunteers, which included both smokers and non-smokers. Blood and BALF neutrophils were identified by CD16(bright)/CD45(dim) cells, and triple-stained with antibodies directed against integrins, chemokine- and Fc gamma-receptors. BALF neutrophils of healthy volunteers showed an activated phenotype characterized by Mac-1 (CD11b)(bright), L-selectin (CD62L)(dim), intercellular adhesion molecule 1 (ICAM-1) (CD54)(bright), Fc gamma RII (CD32)(bright), C5a receptor (CD88)(bright) and CD66b(bright). A similar phenotype was observed for BALF neutrophils of patients affected by sarcoidosis. Furthermore, our results demonstrate a modulated expression of C5a receptor (CD88) and ICAM-1 (CD54) in neutrophils of sarcoidosis patients. In conclusion, our data indicate that neutrophils found in the lung exhibit an activated phenotype under both homeostatic and inflammatory conditions.

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References

Peros-Golubicic T, Ivicevic A, Bekic A, Alilovic M, Tekavec-Trkanjec J, Smojver-Jezek S . Lung lavage neutrophils, neutrophil elastase and albumin in the prognosis of pulmonary sarcoidosis. Coll Antropol. 2002; 25(1):349-55. View

Car B, Meloni F, Luisetti M, Semenzato G, GIALDRONI-GRASSI G, Walz A . Elevated IL-8 and MCP-1 in the bronchoalveolar lavage fluid of patients with idiopathic pulmonary fibrosis and pulmonary sarcoidosis. Am J Respir Crit Care Med. 1994; 149(3 Pt 1):655-9. DOI: 10.1164/ajrccm.149.3.8118632. View

Roebuck K, Finnegan A . Regulation of intercellular adhesion molecule-1 (CD54) gene expression. J Leukoc Biol. 1999; 66(6):876-88. DOI: 10.1002/jlb.66.6.876. View

Ryder M, Fujitaki R, Lebus S, Mahboub M, Faia B, Muhaimin D . Alterations of neutrophil L-selectin and CD18 expression by tobacco smoke: implications for periodontal diseases. J Periodontal Res. 1998; 33(6):359-68. DOI: 10.1111/j.1600-0765.1998.tb02211.x. View

Hietbrink F, Oudijk E, Braams R, Koenderman L, Leenen L . Aberrant regulation of polymorphonuclear phagocyte responsiveness in multitrauma patients. Shock. 2006; 26(6):558-64. DOI: 10.1097/01.shk.0000233196.40989.78. View

Soejima K, Fujishima S, Nakamura H, Waki Y, Nakamura M, Matsubara H . Downmodulation of IL-8 receptors, type A and type B, on human lung neutrophils in vivo. Am J Physiol. 1997; 273(3 Pt 1):L618-25. DOI: 10.1152/ajplung.1997.273.3.L618. View

Iannuzzi M, Rybicki B, Teirstein A . Sarcoidosis. N Engl J Med. 2007; 357(21):2153-65. DOI: 10.1056/NEJMra071714. View

Takabayshi K, Corr M, Hayashi T, Redecke V, Beck L, Guiney D . Induction of a homeostatic circuit in lung tissue by microbial compounds. Immunity. 2006; 24(4):475-87. DOI: 10.1016/j.immuni.2006.02.008. View

Buckley C, Ross E, McGettrick H, Osborne C, Haworth O, Schmutz C . Identification of a phenotypically and functionally distinct population of long-lived neutrophils in a model of reverse endothelial migration. J Leukoc Biol. 2005; 79(2):303-11. DOI: 10.1189/jlb.0905496. View

10.

Yoon J, Terada A, Kita H . CD66b regulates adhesion and activation of human eosinophils. J Immunol. 2007; 179(12):8454-62. DOI: 10.4049/jimmunol.179.12.8454. View

11.

Mann B, Chung K . Blood neutrophil activation markers in severe asthma: lack of inhibition by prednisolone therapy. Respir Res. 2006; 7:59. PMC: 1458332. DOI: 10.1186/1465-9921-7-59. View

12.

Smalley D, Ley K . L-selectin: mechanisms and physiological significance of ectodomain cleavage. J Cell Mol Med. 2005; 9(2):255-66. PMC: 6740228. DOI: 10.1111/j.1582-4934.2005.tb00354.x. View

13.

Kanters D, ten Hove W, Luijk B, van Aalst C, Schweizer R, Lammers J . Expression of activated Fc gamma RII discriminates between multiple granulocyte-priming phenotypes in peripheral blood of allergic asthmatic subjects. J Allergy Clin Immunol. 2007; 120(5):1073-81. DOI: 10.1016/j.jaci.2007.06.021. View

14.

Georas S, Liu M, Newman W, Beall L, Stealey B, Bochner B . Altered adhesion molecule expression and endothelial cell activation accompany the recruitment of human granulocytes to the lung after segmental antigen challenge. Am J Respir Cell Mol Biol. 1992; 7(3):261-9. DOI: 10.1165/ajrcmb/7.3.261. View

15.

Drent M, Jacobs J, de Vries J, Lamers R, Liem I, Wouters E . Does the cellular bronchoalveolar lavage fluid profile reflect the severity of sarcoidosis?. Eur Respir J. 1999; 13(6):1338-44. DOI: 10.1183/09031936.99.13613459. View

16.

Kaseda M, Kadota J, Mukae H, Kawamoto S, Shukuwa T, Iwashita T . Possible role of L-selectin in T lymphocyte alveolitis in patients with active pulmonary sarcoidosis. Clin Exp Immunol. 2000; 121(1):146-50. PMC: 1905659. DOI: 10.1046/j.1365-2249.2000.01267.x. View

17.

Drent M, Mansour K, Linssen C . Bronchoalveolar lavage in sarcoidosis. Semin Respir Crit Care Med. 2007; 28(5):486-95. DOI: 10.1055/s-2007-991521. View

18.

Ziegenhagen M, Rothe M, Schlaak M, Muller-Quernheim J . Bronchoalveolar and serological parameters reflecting the severity of sarcoidosis. Eur Respir J. 2003; 21(3):407-13. DOI: 10.1183/09031936.03.00010403. View

19.

Takizawa H, Satoh M, Okazaki H, Matsuzaki G, Suzuki N, Ishii A . Increased IL-6 and IL-8 in bronchoalveolar lavage fluids (BALF) from patients with sarcoidosis: correlation with the clinical parameters. Clin Exp Immunol. 1997; 107(1):175-81. PMC: 1904552. DOI: 10.1046/j.1365-2249.1997.d01-905.x. View

20.

Warringa R, Mengelers H, Raaijmakers J, Bruijnzeel P, Koenderman L . Upregulation of formyl-peptide and interleukin-8-induced eosinophil chemotaxis in patients with allergic asthma. J Allergy Clin Immunol. 1993; 91(6):1198-205. DOI: 10.1016/0091-6749(93)90323-8. View