Transcript Profiles Uncover Temporal and Stress-induced Changes of Metabolic Pathways in Germinating Sugar Beet Seeds

Overview

Journal BMC Plant Biol

Publisher Biomed Central

Specialty Biology

Date 2008 Dec 3

PMID 19046420

Citations 4

Authors

Elena Pestsova

Juliane Meinhard

Andreas Menze

Uwe Fischer

Andrea Windhovel

Peter Westhoff

Affiliations

Soon will be listed here.

Abstract

Background: With a cultivation area of 1.75 Mio ha and sugar yield of 16.7 Mio tons in 2006, sugar beet is a crop of great economic importance in Europe. The productivity of sugar beet is determined significantly by seed vigour and field emergence potential; however, little is known about the molecular mechanisms underlying these traits. Both traits exhibit large variations within sugar beet germplasm that have been difficult to ascribe to either environmental or genetic causes. Among potential targets for trait improvement, an enhancement of stress tolerance is considered because of the high negative influence of environmental stresses on trait parameters. Extending our knowledge of genetic and molecular determinants of sugar beet germination, stress response and adaptation mechanisms would facilitate the detection of new targets for breeding crop with an enhanced field emergence potential.

Results: To gain insight into the sugar beet germination we initiated an analysis of gene expression in a well emerging sugar beet hybrid showing high germination potential under various environmental conditions. A total of 2,784 ESTs representing 2,251 'unigenes' was generated from dry mature and germinating seeds. Analysis of the temporal expression of these genes during germination under non-stress conditions uncovered drastic transcriptional changes accompanying a shift from quiescent to metabolically active stages of the plant life cycle. Assay of germination under stressful conditions revealed 157 genes showing significantly different expression patterns in response to stress. As deduced from transcriptome data, stress adaptation mechanisms included an alteration in reserve mobilization pathways, an accumulation of the osmoprotectant glycine betaine, late embryogenesis abundant proteins and detoxification enzymes. The observed transcriptional changes are supposed to be regulated by ABA-dependent signal transduction pathway.

Conclusion: This study provides an important step toward the understanding of main events and metabolic pathways during germination in sugar beet. The reported alterations of gene expression in response to stress shed light on sugar beet stress adaptation mechanisms. Some of the identified stress-responsive genes provide a new potential source for improvement of sugar beet stress tolerance during germination and field emergence.

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References

de Los Reyes B, McGrath J . Cultivar-specific seedling vigor and expression of a putative oxalate oxidase germin-like protein in sugar beet (Beta vulgaris L.). Theor Appl Genet. 2003; 107(1):54-61. DOI: 10.1007/s00122-003-1229-9. View

He C, Yan J, Shen G, Fu L, Holaday A, Auld D . Expression of an Arabidopsis vacuolar sodium/proton antiporter gene in cotton improves photosynthetic performance under salt conditions and increases fiber yield in the field. Plant Cell Physiol. 2005; 46(11):1848-54. DOI: 10.1093/pcp/pci201. View

Rajjou L, Gallardo K, Debeaujon I, Vandekerckhove J, Job C, Job D . The effect of alpha-amanitin on the Arabidopsis seed proteome highlights the distinct roles of stored and neosynthesized mRNAs during germination. Plant Physiol. 2004; 134(4):1598-613. PMC: 419834. DOI: 10.1104/pp.103.036293. View

Holdsworth M, Finch-Savage W, Grappin P, Job D . Post-genomics dissection of seed dormancy and germination. Trends Plant Sci. 2007; 13(1):7-13. DOI: 10.1016/j.tplants.2007.11.002. View

Ramanjulu S, Bartels D . Drought- and desiccation-induced modulation of gene expression in plants. Plant Cell Environ. 2002; 25(2):141-151. DOI: 10.1046/j.0016-8025.2001.00764.x. View

Gallardo K, Firnhaber C, Zuber H, Hericher D, Belghazi M, Henry C . A combined proteome and transcriptome analysis of developing Medicago truncatula seeds: evidence for metabolic specialization of maternal and filial tissues. Mol Cell Proteomics. 2007; 6(12):2165-79. DOI: 10.1074/mcp.M700171-MCP200. View

Zhang H, Sreenivasulu N, Weschke W, Stein N, Rudd S, Radchuk V . Large-scale analysis of the barley transcriptome based on expressed sequence tags. Plant J. 2004; 40(2):276-90. DOI: 10.1111/j.1365-313X.2004.02209.x. View

Quan R, Shang M, Zhang H, Zhao Y, Zhang J . Engineering of enhanced glycine betaine synthesis improves drought tolerance in maize. Plant Biotechnol J. 2006; 2(6):477-86. DOI: 10.1111/j.1467-7652.2004.00093.x. View

Koornneef M, Bentsink L, Hilhorst H . Seed dormancy and germination. Curr Opin Plant Biol. 2002; 5(1):33-6. DOI: 10.1016/s1369-5266(01)00219-9. View

10.

Herwig R, Schulz B, Weisshaar B, Hennig S, Steinfath M, Drungowski M . Construction of a 'unigene' cDNA clone set by oligonucleotide fingerprinting allows access to 25 000 potential sugar beet genes. Plant J. 2002; 32(5):845-57. DOI: 10.1046/j.1365-313x.2002.01457.x. View

11.

Fujita Y, Fujita M, Satoh R, Maruyama K, Parvez M, Seki M . AREB1 is a transcription activator of novel ABRE-dependent ABA signaling that enhances drought stress tolerance in Arabidopsis. Plant Cell. 2005; 17(12):3470-88. PMC: 1315382. DOI: 10.1105/tpc.105.035659. View

12.

Lu C, Lin C, Lee K, Chen J, Huang L, Ho S . The SnRK1A protein kinase plays a key role in sugar signaling during germination and seedling growth of rice. Plant Cell. 2007; 19(8):2484-99. PMC: 2002608. DOI: 10.1105/tpc.105.037887. View

13.

Lopez C, Banowetz G, Peterson C, Kronstad W . Differential accumulation of a 24-kd dehydrin protein in wheat seedlings correlates with drought stress tolerance at grain filling. Hereditas. 2002; 135(2-3):175-81. DOI: 10.1111/j.1601-5223.2001.00175.x. View

14.

Hajheidari M, Abdollahian-Noghabi M, Askari H, Heidari M, Sadeghian S, Ober E . Proteome analysis of sugar beet leaves under drought stress. Proteomics. 2005; 5(4):950-60. DOI: 10.1002/pmic.200401101. View

15.

Xu R, Li Q . A RING-H2 zinc-finger protein gene RIE1 is essential for seed development in Arabidopsis. Plant Mol Biol. 2004; 53(1-2):37-50. DOI: 10.1023/b:plan.0000009256.01620.a6. View

16.

Sunkar R, Kapoor A, Zhu J . Posttranscriptional induction of two Cu/Zn superoxide dismutase genes in Arabidopsis is mediated by downregulation of miR398 and important for oxidative stress tolerance. Plant Cell. 2006; 18(8):2051-65. PMC: 1533975. DOI: 10.1105/tpc.106.041673. View

17.

Nomura T, Ueno M, Yamada Y, Takatsuto S, Takeuchi Y, Yokota T . Roles of brassinosteroids and related mRNAs in pea seed growth and germination. Plant Physiol. 2007; 143(4):1680-8. PMC: 1851827. DOI: 10.1104/pp.106.093096. View

18.

Buchanan C, Lim S, Salzman R, Kagiampakis I, Morishige D, Weers B . Sorghum bicolor's transcriptome response to dehydration, high salinity and ABA. Plant Mol Biol. 2005; 58(5):699-720. DOI: 10.1007/s11103-005-7876-2. View

19.

Hu H, Dai M, Yao J, Xiao B, Li X, Zhang Q . Overexpressing a NAM, ATAF, and CUC (NAC) transcription factor enhances drought resistance and salt tolerance in rice. Proc Natl Acad Sci U S A. 2006; 103(35):12987-92. PMC: 1559740. DOI: 10.1073/pnas.0604882103. View

20.

Catusse J, Strub J, Job C, Van Dorsselaer A, Job D . Proteome-wide characterization of sugarbeet seed vigor and its tissue specific expression. Proc Natl Acad Sci U S A. 2008; 105(29):10262-7. PMC: 2474813. DOI: 10.1073/pnas.0800585105. View