In Vitro Analog of Human Bone Marrow from 3D Scaffolds with Biomimetic Inverted Colloidal Crystal Geometry

Overview

Journal Biomaterials

Specialty Biomedical Engineering

Date 2008 Dec 2

PMID 19042018

Citations 38

Authors

Joan E Nichols

Joaquin Cortiella

Jungwoo Lee

Jean A Niles

Meghan Cuddihy

Shaopeng Wang

Joseph Bielitzki

Andrea Cantu

Ron Mlcak

Esther Valdivia

Ryan Yancy

Matthew L McClure

Nicholas A Kotov

Affiliations

Soon will be listed here.

Abstract

In vitro replicas of bone marrow can potentially provide a continuous source of blood cells for transplantation and serve as a laboratory model to examine human immune system dysfunctions and drug toxicology. Here we report the development of an in vitro artificial bone marrow based on a 3D scaffold with inverted colloidal crystal (ICC) geometry mimicking the structural topology of actual bone marrow matrix. To facilitate adhesion of cells, scaffolds were coated with a layer of transparent nanocomposite. After seeding with hematopoietic stem cells (HSCs), ICC scaffolds were capable of supporting expansion of CD34+ HSCs with B-lymphocyte differentiation. Three-dimensional organization was shown to be critical for production of B cells and antigen-specific antibodies. Functionality of bone marrow constructs was confirmed by implantation of matrices containing human CD34+ cells onto the backs of severe combined immunodeficiency (SCID) mice with subsequent generation of human immune cells.

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