The Coordination of Centromere Replication, Spindle Formation, and Kinetochore-microtubule Interaction in Budding Yeast

Overview

Journal PLoS Genet

Specialty Genetics

Date 2008 Nov 22

PMID 19023403

Citations 22

Authors

Hong Liu

Fengshan Liang

Fengzhi Jin

Yanchang Wang

Affiliations

Soon will be listed here.

Abstract

The kinetochore is a protein complex that assembles on centromeric DNA to mediate chromosome-microtubule interaction. Most eukaryotic cells form the spindle and establish kinetochore-microtubule interaction during mitosis, but budding yeast cells finish these processes in S-phase. It has long been noticed that the S-phase spindle in budding yeast is shorter than that in metaphase, but the biological significance of this short S-phase spindle structure remains unclear. We addressed this issue by using ask1-3, a temperature-sensitive kinetochore mutant that exhibits partially elongated spindles at permissive temperature in the presence of hydroxyurea (HU), a DNA synthesis inhibitor. After exposure to and removal of HU, ask1-3 cells show a delayed anaphase entry. This delay depends on the spindle checkpoint, which monitors kinetochore-microtubule interaction defects. Overproduction of microtubule-associated protein Ase1 or Cin8 also induces spindle elongation in HU-arrested cells. The spindle checkpoint-dependent anaphase entry delay is also observed after ASE1 or CIN8 overexpression in HU-arrested cells. Therefore, the shorter spindle in S-phase cells is likely to facilitate proper chromosome-microtubule interaction.

Citing Articles

Timing of Chromosome DNA Integration throughout the Yeast Cell Cycle.

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Yeast Kinesin-5 Motor Protein CIN8 Promotes Accurate Chromosome Segregation.

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Cell-cycle phospho-regulation of the kinetochore.

Klemm C, Thorpe P, Olafsson G Curr Genet. 2020; 67(2):177-193.

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Inhibition of spindle extension through the yeast S phase checkpoint is coupled to replication fork stability and the integrity of centromeric DNA.

Julius J, Peng J, McCulley A, Caridi C, Arnak R, See C Mol Biol Cell. 2019; 30(22):2771-2789.

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