Rapid Saliva Processing Techniques for Near Real-time Analysis of Salivary Steroids and Protein

Overview

Journal J Clin Lab Anal

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
Pathology

Date 2008 Nov 21

PMID 19021269

Citations 3

Authors

Kelly R Atkinson

Kim R Lo

Steve R Payne

John S Mitchell

John R Ingram

Affiliations

Soon will be listed here.

Abstract

Introduction: Point-of-care (POC) measurements using saliva samples have immense potential to assess systemic health and wellbeing, but sample viscosity and contaminants can affect analyses. We sought a portable clean-up method for whole saliva appropriate for use with POC measurement techniques such as biosensors.

Methods: Whole saliva from each of 13 male subjects was split into 5 fractions. Each fraction was treated with a different clean-up process: a freeze-thaw-centrifuge (FTC) step; centrifugation alone; or passage through a Mini-UniPrep polyethersulfone filter, cotton Salivette, or foam Oracol device. Following clean-up, each subject's treated saliva fractions were assayed for cortisol, testosterone, dehydroepiandrosterone (DHEA), and protein concentrations. The effects of clean-up methods on nonspecific binding (NSB) in a biosensor were also assessed.

Results: Compared with FTC, no analytes were affected by centrifugation alone. Cotton Salivettes significantly altered all analytes, with increases in cortisol (+64%), testosterone (+126%), and DHEA (off-scale) levels, and decreased protein (-21%) and biosensor NSB (-75%). Oracol foam devices decreased DHEA levels by 28%. Mini-UniPrep filtration decreased testosterone (-45%) and DHEA (-66%) concentrations while increasing cortisol (+40%).

Conclusion: No method was optimal for all analytes, highlighting the need for validation of saliva treatment methods before their adoption in rapid POC analyses.

Citing Articles

Effects of filtration and alpha-amylase depletion on salivary biochemical composition measurements.

Franco-Martinez L, Ceron J, Martinez-Subiela S, Tvarijonaviciute A PLoS One. 2023; 18(5):e0286092.

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Pretreatment Methods for Human Nasopharyngeal Swabs to Increase the Signal to Noise Ratio of High Sensitivity Immunoassays.

Chen H, Feng S, Zhou W, Li Z, Richard-Greenblatt M, Wang P ACS Meas Sci Au. 2023; 2(5):414-421.

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