A Magic Triangle for Experimental Phasing of Macromolecules

Overview

Journal Acta Crystallogr D Biol Crystallogr

Specialty Chemistry

Date 2008 Nov 21

PMID 19020357

Citations 36

Authors

Tobias Beck

Andrius Krasauskas

Tim Gruene

George M Sheldrick

Affiliations

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Abstract

Obtaining phase information for the solution of macromolecular structures is still one of the bottlenecks in X-ray crystallography. 5-Amino-2,4,6-triiodoisophthalic acid (I3C), in which three covalently bound iodines form an equilateral triangle, was incorporated into proteins in order to obtain phases by single-wavelength anomalous dispersion (SAD). An improved binding capability compared with simple heavy-metal ions, ready availability, improved recognition of potential heavy-atom sites and low toxicity make I3C particularly suitable for experimental phasing.

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