Stabilization of Clathrin Coated Vesicles by Amantadine, Tromantadine and Other Hydrophobic Amines

Overview

Journal FEBS Lett

Specialty Biochemistry

Date 1991 Apr 9

PMID 1901801

Citations 29

Authors

Y Phonphok

K S Rosenthal

Affiliations

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Abstract

Amantadine and related compounds stabilized the structure of purified pig brain clathrin coated vesicles (CCV) at biologically relevant concentrations. Incubation of purified CCV for 30 min at 25 degrees C or 37 degrees C caused the release of clathrin, as determined by a centrifugation assay, and a reduction in the number of coated vesicles, by electron microscopy. Amantadine (10 mM), tromantadine (1 mM), amidine D295 (cyclohexylcarboximidamide-(N-benzyl)hydrochloride (10 mM), chloroquine (0.1 mM) and monodansylcadaverine (10 mM) significantly reduced the extent of dissociation.

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