Small-intestinal Manifestations of Dextran Sulfate Sodium Consumption in Rats and Assessment of the Effects of Lactobacillus Fermentum BR11

Overview

Journal Dig Dis Sci

Specialty Gastroenterology

Date 2008 Nov 14

PMID 19005763

Citations 14

Authors

Mark S Geier

Cassie L Smith

Ross N Butler

Gordon S Howarth

Affiliations

Soon will be listed here.

Abstract

The dextran sulfate sodium (DSS) colitis model has been utilized to screen for novel therapeutics for ulcerative colitis. Evidence suggests the small intestine may also be affected by DSS. We characterized the effects of DSS on the small intestine and assessed the potential for Lactobacillus fermentum BR11 to modify or normalize DSS-induced changes. Rats were allocated to three groups, Water + Vehicle, DSS + Vehicle, and DSS + L. fermentum BR11. BR11 was administered twice daily for 14 days. DSS (2%) was provided from days 7 to 14. Small-intestinal tissue was analyzed for sucrase activity, histology, and crypt cell proliferation. Increased ileum crypt depth and cell proliferation was observed in DSS-treated rats compared to controls (P < 0.05). BR11 normalized these parameters. While DSS predominantly induces colonic damage, minor morphological alterations were also detected in the distal small intestine. L. fermentum BR11 normalized these features.

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