The Bacillus Subtilis Spo0J Gene: Evidence for Involvement in Catabolite Repression of Sporulation

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1991 Mar 1

PMID 1900505

Citations 15

Authors

T H Mysliwiec

J Errington

A B Vaidya

M G Bramucci

Affiliations

Soon will be listed here.

Abstract

Previous observations concerning the ability of the Bacillus subtilis bacteriophages SP10 and PMB12 to suppress mutations in spo0J and to make wild-type sporulation catabolite resistant suggested that spo0J had a role in catabolite repression of sporulation. This suggestion was supported in the present report by the ability of the catabolite-resistant sporulation mutation crsF4 to suppress a Tn917 insertion mutation of the B. subtilis spo0J locus (spo0J::Tn917 omega HU261) in medium without glucose. Although crsF4 and SP10 made wild-type B. subtilis sporulation catabolite resistant, neither crsF4 nor SP10 caused a mutant with spo0J::Tn917 omega HU261 to sporulate in medium with glucose. Sequencing the spo0J locus revealed an open reading frame that was 179 codons in length. Disruption of the open reading frame resulted in a sporulation-negative (Spo-) phenotype that was similar to those of other spo0J mutations. Analysis of the deduced amino acid sequence of the spo0J locus indicated that the spo0J gene product contains an alpha-helix-turn-alpha-helix unit similar to the motif found in lambda Cro-like DNA-binding proteins.

Citing Articles

Regulating Pathways of Adamalysin-like Metalloendopeptidase Expression.

Rudakova N, Sabirova A, Khasanov D, Danilova I, Sharipova M Int J Mol Sci. 2024; 25(1).

PMID: 38203233 PMC: 10779165. DOI: 10.3390/ijms25010062.

Connecting the dots: key insights on ParB for chromosome segregation from single-molecule studies.

Tisma M, Kaljevic J, Gruber S, Le T, Dekker C FEMS Microbiol Rev. 2023; 48(1).

PMID: 38142222 PMC: 10786196. DOI: 10.1093/femsre/fuad067.

Mechanisms for Chromosome Segregation in Bacteria.

Gogou C, Japaridze A, Dekker C Front Microbiol. 2021; 12:685687.

PMID: 34220773 PMC: 8242196. DOI: 10.3389/fmicb.2021.685687.

Bacterial chromosome segregation by the ParABS system.

Jalal A, Le T Open Biol. 2020; 10(6):200097.

PMID: 32543349 PMC: 7333895. DOI: 10.1098/rsob.200097.

Toll-like receptors 1 and 2 heterodimers alter Borrelia burgdorferi gene expression in mice and ticks.

Fikrig E, Narasimhan S, Neelakanta G, Pal U, Chen M, Flavell R J Infect Dis. 2009; 200(8):1331-40.

PMID: 19754309 PMC: 2846271. DOI: 10.1086/605950.

References

Kawamura F, Wang L, Doi R . Catabolite-resistant sporulation (crsA) mutations in the Bacillus subtilis RNA polymerase sigma 43 gene (rpoD) can suppress and be suppressed by mutations in spo0 genes. Proc Natl Acad Sci U S A. 1985; 82(23):8124-8. PMC: 391455. DOI: 10.1073/pnas.82.23.8124. View

Sandman K, Losick R, Youngman P . Genetic analysis of Bacillus subtilis spo mutations generated by Tn917-mediated insertional mutagenesis. Genetics. 1987; 117(4):603-17. PMC: 1203235. DOI: 10.1093/genetics/117.4.603. View

Hoch J, Trach K, Kawamura F, Saito H . Identification of the transcriptional suppressor sof-1 as an alteration in the spo0A protein. J Bacteriol. 1985; 161(2):552-5. PMC: 214917. DOI: 10.1128/jb.161.2.552-555.1985. View

Moriya S, Ogasawara N, Yoshikawa H . Structure and function of the region of the replication origin of the Bacillus subtilis chromosome. III. Nucleotide sequence of some 10,000 base pairs in the origin region. Nucleic Acids Res. 1985; 13(7):2251-65. PMC: 341153. DOI: 10.1093/nar/13.7.2251. View

Trach K, Chapman J, Piggot P, Hoch J . Deduced product of the stage 0 sporulation gene spo0F shares homology with the Spo0A, OmpR, and SfrA proteins. Proc Natl Acad Sci U S A. 1985; 82(21):7260-4. PMC: 390829. DOI: 10.1073/pnas.82.21.7260. View

FERRARI F, Trach K, Lecoq D, Spence J, Ferrari E, Hoch J . Characterization of the spo0A locus and its deduced product. Proc Natl Acad Sci U S A. 1985; 82(9):2647-51. PMC: 397621. DOI: 10.1073/pnas.82.9.2647. View

Clarke S, MANDELSTAM J . Regulation of stage II of sporulation in Bacillus subtilis. J Gen Microbiol. 1987; 133(9):2371-80. DOI: 10.1099/00221287-133-9-2371. View

Losick R, Youngman P, Piggot P . Genetics of endospore formation in Bacillus subtilis. Annu Rev Genet. 1986; 20:625-69. DOI: 10.1146/annurev.ge.20.120186.003205. View

Errington J, Jones D . Cloning in Bacillus subtilis by transfection with bacteriophage vector phi 105J27: isolation and preliminary characterization of transducing phages for 23 sporulation loci. J Gen Microbiol. 1987; 133(3):493-502. DOI: 10.1099/00221287-133-3-493. View

10.

Errington J, MANDELSTAM J . Use of a lacZ gene fusion to determine the dependence pattern of sporulation operon spoIIA in spo mutants of Bacillus subtilis. J Gen Microbiol. 1986; 132(11):2967-76. DOI: 10.1099/00221287-132-11-2967. View

11.

Errington J . A general method for fusion of the Escherichia coli lacZ gene to chromosomal genes in Bacillus subtilis. J Gen Microbiol. 1986; 132(11):2953-66. DOI: 10.1099/00221287-132-11-2953. View

12.

Stock J, Ninfa A, Stock A . Protein phosphorylation and regulation of adaptive responses in bacteria. Microbiol Rev. 1989; 53(4):450-90. PMC: 372749. DOI: 10.1128/mr.53.4.450-490.1989. View

13.

Yamashita S, Kawamura F, Yoshikawa H, Takahashi H, Kobayashi Y, Saito H . Dissection of the expression signals of the spoA gene of Bacillus subtilis: glucose represses sporulation-specific expression. J Gen Microbiol. 1989; 135(5):1335-45. DOI: 10.1099/00221287-135-5-1335. View

14.

Perego M, Cole S, Burbulys D, Trach K, Hoch J . Characterization of the gene for a protein kinase which phosphorylates the sporulation-regulatory proteins Spo0A and Spo0F of Bacillus subtilis. J Bacteriol. 1989; 171(11):6187-96. PMC: 210488. DOI: 10.1128/jb.171.11.6187-6196.1989. View

15.

DUBNAU E, Weir J, Nair G, Carter 3rd L, Moran Jr C, Smith I . Bacillus sporulation gene spo0H codes for sigma 30 (sigma H). J Bacteriol. 1988; 170(3):1054-62. PMC: 210873. DOI: 10.1128/jb.170.3.1054-1062.1988. View

16.

Bouvier J, Stragier P, Bonamy C, SZULMAJSTER J . Nucleotide sequence of the spo0B gene of Bacillus subtilis and regulation of its expression. Proc Natl Acad Sci U S A. 1984; 81(22):7012-6. PMC: 392066. DOI: 10.1073/pnas.81.22.7012. View

17.

Theophilus B, Thomas C . Nucleotide sequence of the transcriptional repressor gene korB which plays a key role in regulation of the copy number of broad host range plasmid RK2. Nucleic Acids Res. 1987; 15(18):7443-50. PMC: 306259. DOI: 10.1093/nar/15.18.7443. View

18.

Kornacki J, Balderes P, Figurski D . Nucleotide sequence of korB, a replication control gene of broad host-range plasmid RK2. J Mol Biol. 1987; 198(2):211-22. DOI: 10.1016/0022-2836(87)90307-x. View

19.

Dodd I, Egan J . Systematic method for the detection of potential lambda Cro-like DNA-binding regions in proteins. J Mol Biol. 1987; 194(3):557-64. DOI: 10.1016/0022-2836(87)90681-4. View

20.

FERRARI F, Trach K, Hoch J . Sequence analysis of the spo0B locus reveals a polycistronic transcription unit. J Bacteriol. 1985; 161(2):556-62. PMC: 214918. DOI: 10.1128/jb.161.2.556-562.1985. View