Identity Tests: Determination of Cell Line Cross-contamination

Overview

Journal Cytotechnology

Specialties Biotechnology
Genetics

Date 2008 Nov 13

PMID 19002894

Citations 15

Authors

C M Cabrera

F Cobo

A Nieto

J L Cortes

R M Montes

P Catalina

A Concha

Affiliations

Soon will be listed here.

Abstract

Cell line cross-contamination is a phenomenon that arises as a result of the continuous cell line culture. It has been estimated that around 20% of the cell lines are misidentified, therefore it is necessary to carry out quality control tests for the detection of this issue. Since cell line cross-contamination discovery, different methods have been applied, such as isoenzyme analysis for inter-species cross-contamination; HLA typing, and DNA fingerprinting using short tandem repeat and a variable number of tandem repeat for intra-species cross-contamination. The cell banks in this sense represent the organizations responsible for guaranteeing the authenticity of cell lines for future research and clinical uses.

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