Expansion of Human Hematopoietic Stem Cells for Transplantation: Trends and Perspectives

Overview

Journal Cytotechnology

Specialties Biotechnology
Genetics

Date 2008 Nov 13

PMID 19002853

Citations 11

Authors

Hera Andrade-Zaldivar

Leticia Santos

Antonio De Leon Rodriguez

Affiliations

Soon will be listed here.

Abstract

Umbilical cord blood transplantation is clinically limited by its low progenitor cell content. Ex vivo expansion has become an alternative to increase the cell dose available for transplants. Expansion has been evaluated in several ways such as static cultures combining growth factors or mimicking the natural microenvironment using co-culture systems. However, static cultures have a small volume capacity and therefore large-scale expansion has been addressed using bioreactors. These and other biotechnological approaches for the expansion of hematopoietic progenitors and their utility to study several aspects of hematopoietic stem cell biology are discussed here.

Citing Articles

Human Hematopoietic Stem Cells Co-cultured in 3D with Stromal Support to Optimize Lentiviral Vector-mediated Gene Transduction.

Kojabad A, Esmaeili Gouvarchin Ghaleh H, Shahriary A, Farzanehpour M Indian J Hematol Blood Transfus. 2023; 39(2):173-182.

PMID: 37006970 PMC: 10064360. DOI: 10.1007/s12288-022-01576-4.

Cord Blood Plasma and Placental Mesenchymal Stem Cells-Derived Exosomes Increase Ex Vivo Expansion of Human Cord Blood Hematopoietic Stem Cells While Maintaining Their Stemness.

Teleb R, Abdul-Hafez A, Othman A, Ahmed A, Elsaid A, Arif H Cells. 2023; 12(2).

PMID: 36672185 PMC: 9857343. DOI: 10.3390/cells12020250.

3D Scaffolds to Model the Hematopoietic Stem Cell Niche: Applications and Perspectives.

Congrains A, Bianco J, Rosa R, Mancuso R, Saad S Materials (Basel). 2021; 14(3).

PMID: 33530372 PMC: 7865713. DOI: 10.3390/ma14030569.

Synergistic Integration of Mesenchymal Stem Cells and Hydrostatic Pressure in the Expansion and Maintenance of Human Hematopoietic/Progenitor Cells.

Kang Y, Jeong J, Lee T, Lee H, Shin J Stem Cells Int. 2018; 2018:4527929.

PMID: 29681947 PMC: 5848107. DOI: 10.1155/2018/4527929.

Ex-vivo expansion of nonhuman primate CD34 cells by stem cell factor Sall4B.

Shen B, Zhang Y, Dai W, Ma Y, Jiang Y Stem Cell Res Ther. 2016; 7(1):152.

PMID: 27765075 PMC: 5072326. DOI: 10.1186/s13287-016-0413-1.

References

Saulnier N, Di Campli C, Zocco M, Di Gioacchino G, Novi M, Gasbarrini A . From stem cell to solid organ. Bone marrow, peripheral blood or umbilical cord blood as favorable source?. Eur Rev Med Pharmacol Sci. 2006; 9(6):315-24. View

Mohamed A, Ibrahim A, El-Masry M, Mansour I, Khroshied M, Gouda H . Ex vivo expansion of stem cells: defining optimum conditions using various cytokines. Lab Hematol. 2006; 12(2):86-93. DOI: 10.1532/LH96.05033. View

Yang S, Cai H, Jin H, Tan W . Hematopoietic reconstitution of CD34+ cells grown in static and stirred culture systems in NOD/SCID mice. Biotechnol Lett. 2007; 30(1):61-5. DOI: 10.1007/s10529-007-9517-0. View

Muller-Sieburg C, Sieburg H . The GOD of hematopoietic stem cells: a clonal diversity model of the stem cell compartment. Cell Cycle. 2006; 5(4):394-8. PMC: 1464375. DOI: 10.4161/cc.5.4.2487. View

Zubler R . Ex vivo expansion of hematopoietic stem cells and gene therapy development. Swiss Med Wkly. 2007; 136(49-50):795-9. DOI: 10.4414/smw.2006.11424. View

Yamaguchi M, Hirayama F, Murahashi H, Azuma H, Sato N, Miyazaki H . Ex vivo expansion of human UC blood primitive hematopoietic progenitors and transplantable stem cells using human primary BM stromal cells and human AB serum. Cytotherapy. 2002; 4(2):109-18. DOI: 10.1080/146532402317381811. View

Bornstein R, Flores A, Montalban M, Del Rey M, de la Serna J, Gilsanz F . A modified cord blood collection method achieves sufficient cell levels for transplantation in most adult patients. Stem Cells. 2005; 23(3):324-34. DOI: 10.1634/stemcells.2004-0047. View

Martin Y, Vermette P . Bioreactors for tissue mass culture: design, characterization, and recent advances. Biomaterials. 2005; 26(35):7481-503. DOI: 10.1016/j.biomaterials.2005.05.057. View

Schoemans H, Theunissen K, Maertens J, Boogaerts M, Verfaillie C, Wagner J . Adult umbilical cord blood transplantation: a comprehensive review. Bone Marrow Transplant. 2006; 38(2):83-93. DOI: 10.1038/sj.bmt.1705403. View

10.

Tong J, Gianni A, Siena S, Srour E, Bregni M, Hoffman R . Primitive hematopoietic progenitor cells are present in peripheral blood autografts. Blood Cells. 1994; 20(2-3):351-62; discussion 362-3. View

11.

Jang Y, Jung D, Jung M, Kim D, Yoo K, Sung K . Mesenchymal stem cells feeder layer from human umbilical cord blood for ex vivo expanded growth and proliferation of hematopoietic progenitor cells. Ann Hematol. 2006; 85(4):212-25. DOI: 10.1007/s00277-005-0047-3. View

12.

Li Q, Liu Q, Cai H, Tan W . A comparative gene-expression analysis of CD34+ hematopoietic stem and progenitor cells grown in static and stirred culture systems. Cell Mol Biol Lett. 2006; 11(4):475-87. PMC: 6275641. DOI: 10.2478/s11658-006-0039-x. View

13.

Collins P, Nielsen L, Patel S, Papoutsakis E, Miller W . Characterization of hematopoietic cell expansion, oxygen uptake, and glycolysis in a controlled, stirred-tank bioreactor system. Biotechnol Prog. 1998; 14(3):466-72. DOI: 10.1021/bp980032e. View

14.

Liu Y, Liu T, Fan X, Ma X, Cui Z . Ex vivo expansion of hematopoietic stem cells derived from umbilical cord blood in rotating wall vessel. J Biotechnol. 2006; 124(3):592-601. DOI: 10.1016/j.jbiotec.2006.01.020. View

15.

Koller M, Palsson M, Manchel I, Maher R, Palsson B . Tissue culture surface characteristics influence the expansion of human bone marrow cells. Biomaterials. 1998; 19(21):1963-72. DOI: 10.1016/s0142-9612(98)00101-x. View

16.

Cabrita G, Ferreira B, Lobato da Silva C, Goncalves R, Almeida-Porada G, Cabral J . Hematopoietic stem cells: from the bone to the bioreactor. Trends Biotechnol. 2003; 21(5):233-40. DOI: 10.1016/S0167-7799(03)00076-3. View

17.

Kwon J, Kim B, Kim M, Park H . Suspension culture of hematopoietic stem cells in stirred bioreactors. Biotechnol Lett. 2003; 25(2):179-82. DOI: 10.1023/a:1021994026859. View

18.

Unsworth B, Lelkes P . Growing tissues in microgravity. Nat Med. 1998; 4(8):901-7. DOI: 10.1038/nm0898-901. View

19.

Xie C, Jia B, Xiang Y, Wang L, Wang G, Huang G . Support of hMSCs transduced with TPO/FL genes to expansion of umbilical cord CD34+ cells in indirect co-culture. Cell Tissue Res. 2006; 326(1):101-10. DOI: 10.1007/s00441-006-0203-7. View

20.

Dykstra B, de Haan G . Hematopoietic stem cell aging and self-renewal. Cell Tissue Res. 2007; 331(1):91-101. DOI: 10.1007/s00441-007-0529-9. View