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Distribution of 14C-TNT and Derivatives in Different Biochemical Compartments of Phaseolus Vulgaris

Overview
Journal Environ Sci Pollut Res Int
Publisher Springer
Specialties Environmental Health
Toxicology
Date 2008 Nov 13
PMID 19002633
Citations 7
Authors
C Sens
P Scheidemann
A Klunk
D Werner
Affiliations
Soon will be listed here.
Abstract

(14)C-TNT was used to quantify the uptake rate and metabolic turnover of TNT in Phaseolus vulgaris. Seventeen plants were analysed by a special cell fractionation method with polar and nonpolar solvents and enzymes. We obtained three cytoplasmic fractions and five cell wall derived fractions. The recovery rate was 72% as measured by liquid scintillation counting. (14)C partitioned almost in equal amounts with approximately 50% in the cytoplasm and in the cell wall. The majority of the TNT-metabolites are present in the cytoplasm as was shown by GC/ECD and thin layer chromatography. The(14)C in the cell wall is bound probably resulting in long-term immobilisation of these metabolites. We conclude that plants may also be a model for nitroaromatic turnover and immobilisation in soil components.

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