Ca2(+)-driven [3H]arachidonate Release in Electropermeabilized Human Platelets Shows an Absolute Requirement for MgATP2-

Overview

Journal Biochem J

Specialty Biochemistry

Date 1991 Feb 1

PMID 1899995

Authors

S Patel

M C Scrutton

Affiliations

Soon will be listed here.

Abstract

Addition of micromolar Ca2+ to electropermeabilized human platelets which had been pre-labelled with [3H]arachidonate causes release of 3H only when millimolar concentrations of a nucleoside triphosphate, e.g. ATP, are present in the incubation medium. Addition of millimolar Ca2+ in the absence of ATP, or preincubation with ATP before addition of micromolar Ca2+, fails to cause a significant increase in 3H release. Purine nucleotides are more effective than pyrimidine nucleotides in activating Ca2(+)-driven 3H release. This activation does not appear to involve phosphate transfer, since metabolically stable analogues of ATP, e.g. the beta gamma-imido analogue, are effective in promoting 3H release.

References

Aster R, JANDL J . PLATELET SEQUESTRATION IN MAN. I. METHODS. J Clin Invest. 1964; 43:843-55. PMC: 289563. DOI: 10.1172/JCI104970. View

PICKETT W, Jesse R, Cohen P . Trypsin-induced phospholipase activity in human platelets. Biochem J. 1976; 160(2):405-8. PMC: 1164248. DOI: 10.1042/bj1600405. View

Nakashima S, Hattori H, Shirato L, Takenaka A, Nozawa Y . Differential sensitivity of arachidonic acid release and 1,2-diacylglycerol formation to pertussis toxin, GDP beta S and NaF in saponin-permeabilized human platelets: possible evidence for distinct GTP-binding proteins involving phospholipase C and.... Biochem Biophys Res Commun. 1987; 148(3):971-8. DOI: 10.1016/s0006-291x(87)80227-9. View

Halenda S, Rehm A . Thrombin and C-kinase activators potentiate calcium-stimulated arachidonic acid release in human platelets. Biochem J. 1987; 248(2):471-5. PMC: 1148565. DOI: 10.1042/bj2480471. View

Sweatt J, Connolly T, Cragoe E, Limbird L . Evidence that Na+/H+ exchange regulates receptor-mediated phospholipase A2 activation in human platelets. J Biol Chem. 1986; 261(19):8667-73. View

Kanaji K, Okuma M, Uchino H . Deficient induction of leukotriene synthesis in human neutrophils by lipoxygenase-deficient platelets. Blood. 1986; 67(4):903-8. View

Touqui L, Rothhut B, Shaw A, Fradin A, Vargaftig B . Platelet activation--a role for a 40K anti-phospholipase A2 protein indistinguishable from lipocortin. Nature. 1986; 321(6066):177-80. DOI: 10.1038/321177a0. View

Mahadevappa V, Holub B . Diacylglycerol lipase pathway is a minor source of released arachidonic acid in thrombin-stimulated human platelets. Biochem Biophys Res Commun. 1986; 134(3):1327-33. DOI: 10.1016/0006-291x(86)90395-5. View

Purdon A, Patelunas D, Smith J . Evidence for the release of arachidonic acid through the selective action of phospholipase A2 in thrombin-stimulated human platelets. Biochim Biophys Acta. 1987; 920(3):205-14. DOI: 10.1016/0005-2760(87)90096-8. View

10.

Nakashima S, Suganuma A, Matsui A, Hattori H, Sato M, Takenaka A . Primary role of calcium ions in arachidonic acid release from rat platelet membranes. Comparison with human platelet membranes. Biochem J. 1989; 259(1):139-44. PMC: 1138483. DOI: 10.1042/bj2590139. View

11.

Loeb L, Gross R . Identification and purification of sheep platelet phospholipase A2 isoforms. Activation by physiologic concentrations of calcium ion. J Biol Chem. 1986; 261(23):10467-70. View

12.

Aarsman A, Roosenboom C, van Geffen G, van den Bosch H . Some aspects of rat platelet and serum phospholipase A2 activities. Biochim Biophys Acta. 1985; 837(3):288-95. DOI: 10.1016/0005-2760(85)90052-9. View

13.

Kannagi R, Koizumi K, Masuda T . Limited hydrolysis of platelet membrane phospholipids. On the proposed phospholipase-susceptible domain in platelet membranes. J Biol Chem. 1981; 256(3):1177-84. View

14.

Knight D, Niggli V, Scrutton M . Thrombin and activators of protein kinase C modulate secretory responses of permeabilised human platelets induced by Ca2+. Eur J Biochem. 1984; 143(2):437-46. DOI: 10.1111/j.1432-1033.1984.tb08391.x. View

15.

Knight D, Scrutton M . Direct evidence for a role for Ca2+ in amine storage granule secretion by human platelets. Thromb Res. 1980; 20(4):437-46. DOI: 10.1016/0049-3848(80)90282-0. View

16.

Lagarde M, Guichardant M, Menashi S, Crawford N . The phospholipid and fatty acid composition of human platelet surface and intracellular membranes isolated by high voltage free flow electrophoresis. J Biol Chem. 1982; 257(6):3100-4. View

17.

Jesse R, Franson R . Modulation of purified phospholipase A2 activity from human platelets by calcium and indomethacin. Biochim Biophys Acta. 1979; 575(3):467-70. DOI: 10.1016/0005-2760(79)90117-6. View

18.

Fabiato A, Fabiato F . Calculator programs for computing the composition of the solutions containing multiple metals and ligands used for experiments in skinned muscle cells. J Physiol (Paris). 1979; 75(5):463-505. View

19.

BLIGH E, Dyer W . A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959; 37(8):911-7. DOI: 10.1139/o59-099. View