Greatly Reduced Amino Acid Alphabets in Directed Evolution: Making the Right Choice for Saturation Mutagenesis at Homologous Enzyme Positions

Overview

Journal Chem Commun (Camb)

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2008 Nov 11

PMID 18997931

Citations 29

Authors

Manfred T Reetz

Sheng Wu

Affiliations

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Abstract

Enantioselective mutants of the thermally robust phenyl acetone monooxygenase (PAMO) as catalysts in Baeyer-Villiger reactions have been evolved by utilizing saturation mutagenesis in which drastically reduced amino acid alphabets are employed at homologous enzyme positions.

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