The Ceramide Structure of GM1 Ganglioside Differently Affects Its Recovery in Low-density Membrane Fractions Prepared from HL-60 Cells with or Without Triton-X100

Overview

Journal Cell Mol Biol Lett

Publisher Biomed Central

Specialties Biochemistry
Cell Biology
Molecular Biology

Date 2008 Nov 4

PMID 18979068

Citations 1

Authors

Miroslawa Panasiewicz

Hanna Domek

Grazyna Hoser

Natalia Fedoryszak

Maciej Kawalec

Tadeusz Pacuszka

Affiliations

Soon will be listed here.

Abstract

Gangliosides are characteristically enriched in various membrane domains that can be isolated as low density membrane fraction insoluble in detergents (detergent-resistant membranes, DRMs) or obtained after homogenization and sonication in 0.5 M sodium carbonate (low-density membranes, LDMs). We assessed the effect of the ceramide structure of four [(3)H]-labeled GM1 ganglioside molecular species (GM1s) taken up by HL-60 cells on their occurrence in LDMs, and compared it with our previous observations for DRMs. All GM1s contained C18 sphingosine, which was acetylated in GM1(18:1/2) or acylated with C(14), C(18) or C(18:1) fatty acids (Fas).

Citing Articles

Systematic analyses of free ceramide species and ceramide species comprising neutral glycosphingolipids by MALDI-TOF MS with high-energy CID.

Tanaka K, Yamada M, Tamiya-Koizumi K, Kannagi R, Aoyama T, Hara A Glycoconj J. 2011; 28(2):67-87.

PMID: 21400001 DOI: 10.1007/s10719-011-9325-6.

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