Establishment of CD5 and CD10 Double-positive Mature B-cell Line, WILL1, Showing Complex 8q24 Translocation Involving 14q32 and 6q27

Overview

Journal Int J Hematol

Specialty Hematology

Date 2008 Oct 31

PMID 18972186

Citations 1

Authors

Shima Uneda

Minako Gotoh

Takashi Sonoki

Kazuhiro Nishida

Yasushi Nakamura

Miwa Kurimoto

Nobuyoshi Hanaoka

Hiroshi Matsuoka

Masafumi Taniwaki

Hideki Nakakuma

Affiliations

Soon will be listed here.

Abstract

We established a novel mature B-cell line from a CD5 and CD10 double-positive diffuse large B-cell lymphoma patient, designated as WILL1. WILL1 cells were positive for CD5, CD10, CD19, and CD20. Spectral karyotype (SKY) analysis revealed chromosome 8 signals on 6q27 as well as 14q32. Fluorescence in situ hybridization (FISH) analysis suggested that a translocation break occurred outside the immunoglobulin heavy chain (IGH) gene on 14q32. Moreover, fusion signals of IGH and C-MYC probes were detected on the derivative 6 and derivative 14 chromosomes. Southern blot analysis using a C-MYC exon II fragment failed to detect rearrangement, suggesting that the 8q24 breakpoints lay far up- or downstream of the C-MYC gene. WILL1 is a useful tool to analyze the pathogenesis of CD5 and CD10 double-positive diffuse large B-cell lymphoma, and for molecular cloning of the unique translocation breakpoints of 14q32 and 8q24 and a novel gene on 6q27.

Citing Articles

Bone marrow large B cell lymphoma bearing cyclin D3 expression: clinical, morphologic, immunophenotypic, and genotypic analyses of seven patients.

Watanuki J, Hatakeyama K, Sonoki T, Tatetsu H, Yoshida K, Fujii S Int J Hematol. 2009; 90(2):217-225.

PMID: 19639271 DOI: 10.1007/s12185-009-0382-6.

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