Simultaneous Quantification of Plant Glyceroglycolipids Including Sulfoquinovosyldiacylglycerol by HPLC-ELSD with Binary Gradient Elution

Overview

Journal Lipids

Specialty Biochemistry

Date 2008 Oct 23

PMID 18941820

Citations 8

Authors

Keita Yunoki

Mayumi Sato

Kazuto Seki

Takeshi Ohkubo

Yukihisa Tanaka

Masao Ohnishi

Affiliations

Soon will be listed here.

Abstract

Membrane lipids of photosynthetic organisms consist of glycerophospholipids and glyceroglycolipids. We investigated a method for the simultaneous quantitative analysis of neutral and acidic lipids using HPLC-ELSD, and quantified monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG) and sulfoquinovosyldiacylglycerol (SQDG). Ten complex lipid classes were separated with a binary gradient system consisting of chloroform and methanol-acetone-water-acetic acid (30:60:9:1, v/v/v/v) with 0.3% triethylamine (pH 4), and were eluted within 16 min. The contents of SQDG in ten edible plants ranged from 3 to 101 mg/100 g, and were positively correlated to the neutral glyceroglycolipids contents.

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