Raman Study of Mechanically Induced Oxygenation State Transition of Red Blood Cells Using Optical Tweezers

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 2008 Oct 22

PMID 18931252

Citations 22

Authors

Satish Rao

Stefan Balint

Benjamin Cossins

Victor Guallar

Dmitri Petrov

Affiliations

Soon will be listed here.

Abstract

Raman spectroscopy was used to monitor changes in the oxygenation state of human red blood cells while they were placed under mechanical stress with the use of optical tweezers. The applied force is intended to simulate the stretching and compression that cells experience as they pass through vessels and smaller capillaries. In this work, spectroscopic evidence of a transition between the oxygenation and deoxygenation states, which is induced by stretching the cell with optical tweezers, is presented. The transition is due to enhanced hemoglobin-membrane and hemoglobin neighbor-neighbor interactions, and the latter was further studied by modeling the electrostatic binding of two of the protein structures.

Citing Articles

Comparison of the human's and camel's red blood cell deformability by optical tweezers and Raman spectroscopy.

Pesen T, Haydaroglu M, Capar S, Parlatan U, Unlu M Biochem Biophys Rep. 2023; 35:101490.

PMID: 37664525 PMC: 10474369. DOI: 10.1016/j.bbrep.2023.101490.

The effects of lithium on human red blood cells studied using optical spectroscopy and laser trap.

Singh Y, Chowdhury A, Dasgupta R, Majumder S Eur Biophys J. 2023; 52(1-2):91-100.

PMID: 36929427 DOI: 10.1007/s00249-023-01643-2.

Laser Raman tweezer spectroscopy to explore the bisphenol A-induced changes in human erythrocytes.

Lukose J, N M, M P, Mohan G, Shastry S, Chidangil S RSC Adv. 2022; 9(28):15933-15940.

PMID: 35521407 PMC: 9064284. DOI: 10.1039/c9ra01840a.

Normal saline-induced deoxygenation of red blood cells probed by optical tweezers combined with the micro-Raman technique.

Lukose J, N M, Mohan G, Shastry S, Chidangil S RSC Adv. 2022; 9(14):7878-7884.

PMID: 35521160 PMC: 9061285. DOI: 10.1039/c8ra10061f.

Atomic force microscopy and Raman spectra profile of blood components associated with exposure to cigarette smoking.

Burgara-Estrella A, Acosta-Elias M, Alvarez-Bajo O, Silva-Campa E, Angulo-Molina A, Rodriguez-Hernandez I RSC Adv. 2022; 10(20):11971-11981.

PMID: 35496627 PMC: 9050830. DOI: 10.1039/d0ra01384f.

References

Vera C, Skelton R, Bossens F, Sung L . 3-D nanomechanics of an erythrocyte junctional complex in equibiaxial and anisotropic deformations. Ann Biomed Eng. 2005; 33(10):1387-404. DOI: 10.1007/s10439-005-4698-y. View

De Rosa M, Carelli Alinovi C, Galtieri A, Russo A, Giardina B . Allosteric properties of hemoglobin and the plasma membrane of the erythrocyte: new insights in gas transport and metabolic modulation. IUBMB Life. 2008; 60(2):87-93. DOI: 10.1002/iub.15. View

Katchalski-Katzir E, Shariv I, Eisenstein M, Friesem A, Aflalo C, Vakser I . Molecular surface recognition: determination of geometric fit between proteins and their ligands by correlation techniques. Proc Natl Acad Sci U S A. 1992; 89(6):2195-9. PMC: 48623. DOI: 10.1073/pnas.89.6.2195. View

KREUZER F, YAHR W . Influence of red cell membrane on diffusion of oxygen. J Appl Physiol. 1960; 15:1117-22. DOI: 10.1152/jappl.1960.15.6.1117. View

Alden R, Satterlee J, Mintorovitch J, Constantinidis I, Ondrias M, Swanson B . The effects of high pressure upon ligated and deoxyhemoglobins and myoglobin. An optical spectroscopic study. J Biol Chem. 1989; 264(4):1933-40. View

Grier D . A revolution in optical manipulation. Nature. 2003; 424(6950):810-6. DOI: 10.1038/nature01935. View

De Luca A, Rusciano G, Ciancia R, Martinelli V, Pesce G, Rotoli B . Spectroscopical and mechanical characterization of normal and thalassemic red blood cells by Raman Tweezers. Opt Express. 2008; 16(11):7943-57. DOI: 10.1364/oe.16.007943. View

DeMoll E, Cox D, Daniel E, Riggs A . Apparent specific volume of human hemoglobin: effect of ligand state and contribution of heme. Anal Biochem. 2007; 363(2):196-203. DOI: 10.1016/j.ab.2007.01.035. View

Jay A, Canham P . Viscoelastic properties of the human red blood cell membrane. II. Area and volume of individual red cells entering a micropipette. Biophys J. 1977; 17(2):169-78. PMC: 1473456. DOI: 10.1016/S0006-3495(77)85634-8. View

10.

Sleep J, Wilson D, Simmons R, Gratzer W . Elasticity of the red cell membrane and its relation to hemolytic disorders: an optical tweezers study. Biophys J. 1999; 77(6):3085-95. PMC: 1300579. DOI: 10.1016/S0006-3495(99)77139-0. View

11.

MacDonald M, Spalding G, Dholakia K . Microfluidic sorting in an optical lattice. Nature. 2003; 426(6965):421-4. DOI: 10.1038/nature02144. View

12.

Gabb H, Jackson R, STERNBERG M . Modelling protein docking using shape complementarity, electrostatics and biochemical information. J Mol Biol. 1997; 272(1):106-20. DOI: 10.1006/jmbi.1997.1203. View

13.

Perutz M . Stereochemistry of cooperative effects in haemoglobin. Nature. 1970; 228(5273):726-39. DOI: 10.1038/228726a0. View

14.

Alcantara R, Xu C, Spiro T, Guallar V . A quantum-chemical picture of hemoglobin affinity. Proc Natl Acad Sci U S A. 2007; 104(47):18451-5. PMC: 2141797. DOI: 10.1073/pnas.0706026104. View

15.

Chan J, Taylor D, Lane S, Zwerdling T, Tuscano J, Huser T . Nondestructive identification of individual leukemia cells by laser trapping Raman spectroscopy. Anal Chem. 2008; 80(6):2180-7. DOI: 10.1021/ac7022348. View

16.

Ashkin A, Dziedzic J, Yamane T . Optical trapping and manipulation of single cells using infrared laser beams. Nature. 1987; 330(6150):769-71. DOI: 10.1038/330769a0. View

17.

Cheng T, Blundell T, Fernandez-Recio J . pyDock: electrostatics and desolvation for effective scoring of rigid-body protein-protein docking. Proteins. 2007; 68(2):503-15. DOI: 10.1002/prot.21419. View

18.

Perutz M, Wilkinson A, Paoli M, Dodson G . The stereochemical mechanism of the cooperative effects in hemoglobin revisited. Annu Rev Biophys Biomol Struct. 1998; 27:1-34. DOI: 10.1146/annurev.biophys.27.1.1. View

19.

Venkatesh B, Ramasamy S, Mylrajan M, Asokan R, Manoharan P, Rifkind J . Fourier transform Raman approach to structural correlation in hemoglobin derivatives. Spectrochim Acta A Mol Biomol Spectrosc. 1999; 55A(7-8):1691-7. DOI: 10.1016/s1386-1425(99)00008-6. View

20.

Liu Y, Cheng D, Sonek G, Berns M, Chapman C, Tromberg B . Evidence for localized cell heating induced by infrared optical tweezers. Biophys J. 1995; 68(5):2137-44. PMC: 1282119. DOI: 10.1016/S0006-3495(95)80396-6. View