Clinical Improvement After Treatment with VEGF(165) in Patients with Severe Chronic Lower Limb Ischaemia

Overview

Journal Genomic Med

Publisher Springer

Specialty Genetics

Date 2008 Oct 17

PMID 18923928

Citations 1

Authors

Andrei Anghel

Bogdan Mut-Vitcu

Lorand Savu

Catalin Marian

Edward Seclaman

Raluca Iman

Adriana-Maria Neghina

Stefan I Dragulescu

Affiliations

Soon will be listed here.

Abstract

The present study focuses on the application of a therapeutic strategy in patients with chronic severe lower limb ischaemia using a plasmid vector encoding the vascular endothelial growth factor (phVEGF(165)). It has been shown that VEGF promotes neo-vascularization and blood vessel network formation and thus might have the ability to improve blood-flow at the level of the affected limbs. However, little information is available regarding the necessary level of expression of VEGF and its possible related adverse effects. We have subcloned VEGF ( 165 )isoform into pCMV-Script expression vector (Stratagene) under the control of the CMV promoter. Three patients with chronic ischaemia of the lower limb, considered as not suitable for surgical re-vascularization, received intramuscular injection with 0.5 ml saline solution containing 10(11) copies of VEGF ( 165 ) plasmid. The clinical evolution has been monitored by angiography and estimated by walking time on the rolling carpet (Gardner protocol). Two months after therapy, all three patients showed complete relief of rest pain, improvement of ischaemic ulcer lesions and increased walking distance on the rolling carpet most probably due to appearance of newly formed collateral vessels.

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