Multiplication of Legionella Spp. in Tap Water Containing Hartmannella Vermiformis

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1991 Jul 1

PMID 1892386

Citations 36

Authors

R M Wadowsky

T M Wilson

N J Kapp

A J West

J M Kuchta

S J States

J N Dowling

R B Yee

Affiliations

Soon will be listed here.

Abstract

A model was developed to study the multiplication of various Legionella spp. in tap water containing Hartmannella vermiformis. Tap water cultures prepared with the following components were suitable for the multiplication studies: Legionella spp., 10(3) CFU/ml; H. vermiformis, 10(4.4) cysts per ml; and killed Pseudomonas paucimobilis, 10(9) cells per ml. Cocultures were incubated at 37 degrees C for at least 1 week. The following legionellae multiplied in tap water cocultures in each replicate experiment: L. bozemanii (WIGA strain), L. dumoffii (NY-23 and TX-KL strains), L. micdadei (two environmental strains), and L. pneumophila (six environmental strains and one clinical isolate). Growth yield values for these strains were 0.6 to 3.5 log CFU/ml. Legionellae which did not multiply in replicate cocultures included L. anisa (one strain), L. bozemanii (MI-15 strain), L. micdadei (a clinical isolate), L. longbeachae, (one strain), and L. pneumophila (Philadelphia 1 strain). L. gormanii and an environmental isolate of L. pneumophila multiplied in only one of three experiments. None of the legionellae multiplied in tap water containing only killed P. paucimobilis. The mean growth yield (+/- standard deviation) of H. vermiformis in the cocultures was 1.2 +/- 0.1 log units/ml. H. vermiformis supports multiplication of only particular strains of legionellae, some of which are from diverse origins.

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References

Breiman R, Fields B, Sanden G, Volmer L, Meier A, Spika J . Association of shower use with Legionnaires' disease. Possible role of amoebae. JAMA. 1990; 263(21):2924-6. View

Donowitz G, Reardon I, Dowling J, Rubin L, Focht D . Ingestion of Legionella micdadei inhibits human neutrophil function. Infect Immun. 1990; 58(10):3307-11. PMC: 313654. DOI: 10.1128/iai.58.10.3307-3311.1990. View

Holden E, Winkler H, Wood D, Leinbach E . Intracellular growth of Legionella pneumophila within Acanthamoeba castellanii Neff. Infect Immun. 1984; 45(1):18-24. PMC: 263250. DOI: 10.1128/iai.45.1.18-24.1984. View

Anand C, Skinner A, Malic A, Kurtz J . Interaction of L. pneumophilia and a free living amoeba (Acanthamoeba palestinensis). J Hyg (Lond). 1983; 91(2):167-78. PMC: 2129387. DOI: 10.1017/s0022172400060174. View

Horwitz M . The Legionnaires' disease bacterium (Legionella pneumophila) inhibits phagosome-lysosome fusion in human monocytes. J Exp Med. 1983; 158(6):2108-26. PMC: 2187157. DOI: 10.1084/jem.158.6.2108. View

Horwitz M, Silverstein S . Interaction of the Legionnaires' disease bacterium (Legionella pneumophila) with human phagocytes. I. L. pneumophila resists killing by polymorphonuclear leukocytes, antibody, and complement. J Exp Med. 1981; 153(2):386-97. PMC: 2186085. DOI: 10.1084/jem.153.2.386. View

Horwitz M, Silverstein S . Legionnaires' disease bacterium (Legionella pneumophila) multiples intracellularly in human monocytes. J Clin Invest. 1980; 66(3):441-50. PMC: 371671. DOI: 10.1172/JCI109874. View

Edelstein P . Comparative study of selective media for isolation of Legionella pneumophila from potable water. J Clin Microbiol. 1982; 16(4):697-9. PMC: 272448. DOI: 10.1128/jcm.16.4.697-699.1982. View

Yee R, Wadowsky R . Multiplication of Legionella pneumophila in unsterilized tap water. Appl Environ Microbiol. 1982; 43(6):1330-4. PMC: 244236. DOI: 10.1128/aem.43.6.1330-1334.1982. View

10.

Wadowsky R, Yee R, Mezmar L, Wing E, Dowling J . Hot water systems as sources of Legionella pneumophila in hospital and nonhospital plumbing fixtures. Appl Environ Microbiol. 1982; 43(5):1104-10. PMC: 244193. DOI: 10.1128/aem.43.5.1104-1110.1982. View

11.

Wadowsky R, Yee R . Glycine-containing selective medium for isolation of Legionellaceae from environmental specimens. Appl Environ Microbiol. 1981; 42(5):768-72. PMC: 244105. DOI: 10.1128/aem.42.5.768-772.1981. View

12.

Rowbotham T . Preliminary report on the pathogenicity of Legionella pneumophila for freshwater and soil amoebae. J Clin Pathol. 1980; 33(12):1179-83. PMC: 1146371. DOI: 10.1136/jcp.33.12.1179. View

13.

Pasculle A, Feeley J, Gibson R, Cordes L, Myerowitz R, Patton C . Pittsburgh pneumonia agent: direct isolation from human lung tissue. J Infect Dis. 1980; 141(6):727-32. DOI: 10.1093/infdis/141.6.727. View

14.

CHERRY W, Pittman B, Harris P, HEBERT G, Thomason B, Thacker L . Detection of Legionnaires disease bacteria by direct immunofluorescent staining. J Clin Microbiol. 1978; 8(3):329-38. PMC: 275241. DOI: 10.1128/jcm.8.3.329-338.1978. View

15.

Wadowsky R, Butler L, COOK M, Verma S, Paul M, Fields B . Growth-supporting activity for Legionella pneumophila in tap water cultures and implication of hartmannellid amoebae as growth factors. Appl Environ Microbiol. 1988; 54(11):2677-82. PMC: 204355. DOI: 10.1128/aem.54.11.2677-2682.1988. View

16.

Horwitz M, Maxfield F . Legionella pneumophila inhibits acidification of its phagosome in human monocytes. J Cell Biol. 1984; 99(6):1936-43. PMC: 2113576. DOI: 10.1083/jcb.99.6.1936. View

17.

States S, Conley L, Kuchta J, Oleck B, Lipovich M, Wolford R . Survival and multiplication of Legionella pneumophila in municipal drinking water systems. Appl Environ Microbiol. 1987; 53(5):979-86. PMC: 203798. DOI: 10.1128/aem.53.5.979-986.1987. View

18.

Henke M, Seidel K . Association between Legionella pneumophila and amoebae in water. Isr J Med Sci. 1986; 22(9):690-5. View

19.

Rowbotham T . Current views on the relationships between amoebae, legionellae and man. Isr J Med Sci. 1986; 22(9):678-89. View

20.

Kuchta J, States S, McGlaughlin J, Overmeyer J, Wadowsky R, McNamara A . Enhanced chlorine resistance of tap water-adapted Legionella pneumophila as compared with agar medium-passaged strains. Appl Environ Microbiol. 1985; 50(1):21-6. PMC: 238566. DOI: 10.1128/aem.50.1.21-26.1985. View