Synthesis of Cytochrome C Oxidase Polypeptides in an Escherichia Coli Cell-free System Directed by Saccharomyces Cerevisiae Mitochondrial DNA

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1977 Jan 7

PMID 189180

Citations 1

Authors

A H Scragg

D Y Thomas

Affiliations

Soon will be listed here.

Abstract

Using purified yeast mitochondrial DNA as a template for E. coli RNA polymerase (holoenzyme) complementary mitochondrial RNA has been synthesized in vitro. This RNA has been used to direct a low background E. coli S-30 protein-synthesizing system. The synthesis of mitochondrial polypeptides has been detected by using antiserum raised against purified cytochrome c oxidase holoenzyme and shown to be specific for this antigen. The antiserum-antigen complex was dissociated and subject to SDS-polyacrylamide gel electrophoresis and the presence of 3 polypeptides of 39, 31, and 26 X 10(3) daltons molecular weight demonstrated, which correspond to the subunits synthesized by mitochondria in whole cells which are inhibited with cycloheximide.

Citing Articles

Expression of cloned mitochondrial DNA from the petite negative yeast Schizosaccharomyces pombe in E. coli minicells.

Del Giudice L, Wolf K, Manna F, Massardo D Mol Gen Genet. 1983; 191(1):91-8.

PMID: 6350830 DOI: 10.1007/BF00330895.

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