Increased Production of Oxygen Free Radicals in Cigarette Smokers

Overview

Journal Int J Exp Pathol

Publisher Wiley

Specialty Pathology

Date 1991 Feb 1

PMID 1888662

Citations 41

Authors

J Kalra

A K Chaudhary

K Prasad

Affiliations

Soon will be listed here.

Abstract

Oxygen free radicals are known to produce damage in many biological tissues. Cigarette smoking is a major risk factor for various diseases. It is possible that oxygen free radical producing activity of polymorphonuclear (PMN) leucocytes is increased by cigarette smoking. We studied the oxygen free radical producing (luminol-dependent chemiluminescent) activity of PMN leucocytes in blood and the malondialdehyde (lipid peroxidation product) content of blood and serum in nonsmokers and smokers. The zymosan-induced chemiluminescent activity was measured on a LKB 1251 luminometer. The malondialdehyde (MDA) was measured as thiobarbituric acid (TBA) reactive substances. The chemiluminescent activity due to oxygen-derived free radicals (superoxide anion, hydrogen peroxide, hydroxyl radical) and superoxide dismutase (SOD)-inhibitable (superoxide anion) in nonsmokers were 1215.1 +/- 91.1 and 849.3 +/- 72.3 mV min/10(6) PMN leucocytes respectively. There was a significant increase in the oxygen-derived free radicals and SOD-inhibitable chemiluminescence in smokers. The values of blood and serum MDA were 171.7 +/- 6.1 and 222.2 +/- 5.6 nmoles/l respectively in nonsmokers. There was an increase in both blood and serum MDA in smokers. These results suggest that the increased generation of oxygen free radicals by PMN leucocytes might be responsible for an enhanced risk of various diseases related to cigarette smoking.

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References

DEWALD B, Baggiolini M, Curnutte J, Babior B . Subcellular localization of the superoxide-forming enzyme in human neutrophils. J Clin Invest. 1979; 63(1):21-9. PMC: 371913. DOI: 10.1172/JCI109273. View

Kannel W . Update on the role of cigarette smoking in coronary artery disease. Am Heart J. 1981; 101(3):319-28. DOI: 10.1016/0002-8703(81)90197-6. View

AUERBACH O, CARTER H, GARFINKEL L, HAMMOND E . Smoker's heart. Arch Intern Med. 1977; 137(4):435. View

Nelson R, Herron M, Schmidtke J, Simmons R . Chemiluminescence response of human leukocytes: influence of medium components on light production. Infect Immun. 1977; 17(3):513-20. PMC: 421154. DOI: 10.1128/iai.17.3.513-520.1977. View

Yagi K . A simple fluorometric assay for lipoperoxide in blood plasma. Biochem Med. 1976; 15(2):212-6. DOI: 10.1016/0006-2944(76)90049-1. View

Prasad K, Kalra J, Bharadwaj B . Increased chemiluminescence of polymorphonuclear leucocytes in dogs with volume overload heart failure. Br J Exp Pathol. 1989; 70(4):463-8. PMC: 2040557. View

Bridges R, Wyatt R, Rehm S . Effect of smoking on peripheral blood leukocytes and serum antiproteases. Eur J Respir Dis Suppl. 1985; 139:24-33. View

Ross R . The pathogenesis of atherosclerosis--an update. N Engl J Med. 1986; 314(8):488-500. DOI: 10.1056/NEJM198602203140806. View

Ueno N, Matsumoto T, Ohkawa M, Matsumoto S . Chemiluminescence of whole blood. 1. A simple and rapid method for the estimation of phagocytic function of granulocytes and opsonic activity in whole blood. Clin Immunol Immunopathol. 1983; 26(1):66-75. DOI: 10.1016/0090-1229(83)90174-5. View

10.

Freeman B, Crapo J . Biology of disease: free radicals and tissue injury. Lab Invest. 1982; 47(5):412-26. View

11.

Hoidal J, Niewoehner D . Pathogenesis of emphysema. Chest. 1983; 83(4):679-85. DOI: 10.1378/chest.83.4.679. View

12.

Shingu M, Nobunaga M . Chemotactic activity generated in human serum from the fifth component of complement by hydrogen peroxide. Am J Pathol. 1984; 117(2):201-6. PMC: 1900453. View

13.

Holt M, Ryall M, Campbell A . Albumin inhibits human polymorphonuclear leucocyte luminol-dependent chemiluminescence: evidence for oxygen radical scavenging. Br J Exp Pathol. 1984; 65(2):231-41. PMC: 2040953. View

14.

Hastings M, Petricevic I, Williams A, COLE P, Easmon C . The effect of culture media on the production and measurement of luminol-dependent chemiluminescence. Br J Exp Pathol. 1982; 63(2):147-53. PMC: 2040613. View

15.

Dechatelet L, Shirley P . Evaluation of chronic granulomatous disease by a chemiluminescence assay of microliter quantities of whole blood. Clin Chem. 1981; 27(10):1739-41. View

16.

Anderson D, FERRIS Jr B . Role of tobacco smoking in the causation of chronic respiratory disease. N Engl J Med. 1962; 267:787-94. DOI: 10.1056/NEJM196210182671601. View

17.

AUERBACH O, CARTER H, GARFINKEL L, HAMMOND E . Cigarette smoking and coronary artery disease. A macroscopic and microscopic study. Chest. 1976; 70(6):697-705. DOI: 10.1378/chest.70.6.697. View

18.

Kew R, Ghebrehiwet B, Janoff A . Cigarette smoke can activate the alternative pathway of complement in vitro by modifying the third component of complement. J Clin Invest. 1985; 75(3):1000-7. PMC: 423647. DOI: 10.1172/JCI111760. View

19.

Lucchesi B, Mullane K . Leukocytes and ischemia-induced myocardial injury. Annu Rev Pharmacol Toxicol. 1986; 26:201-24. DOI: 10.1146/annurev.pa.26.040186.001221. View

20.

Webster R, Hong S, JOHNSTON Jr R, Henson P . Biologial effects of the human complement fragments C5a and C5ades Arg on neutrophil function. Immunopharmacology. 1980; 2(3):201-19. DOI: 10.1016/0162-3109(80)90050-8. View