Miniaturization of Three Carbohydrate Analyses Using a Microsample Plate Reader

Overview

Journal Anal Biochem

Publisher Elsevier

Specialty Biochemistry

Date 1991 May 15

PMID 1888021

Citations 53

Authors

J D Fox

J F Robyt

Affiliations

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Abstract

Three carbohydrate analyses (reducing value by copper-bicinchoninate, total carbohydrate by phenol-sulfuric acid, and D-glucose by glucose oxidase) have been miniaturized using a microsample plate reader. The use of the reducing-value procedure to measure the hydrolysis of starch by alpha-amylase and the use of the glucose oxidase method to measure the hydrolysis of lactose by lactase are illustrated.

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