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A Guide to the Perplexed on the Specificity of Antibodies

Overview
Publisher Sage Publications
Specialty Biochemistry
Date 2008 Oct 16
PMID 18854594
Citations 72
Authors
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Abstract

Many investigators are unaware of the potential problems with specificity of antibodies and the need to document antibody characterization meticulously for each antibody that is used. In this review, I consider the principles of antibody action and how they define a set of rules for what information should be obtained by the investigator before using an antibody in a serious scientific investigation.

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References
1.
Fox C, Johnson F, Whiting J, Roller P . Formaldehyde fixation. J Histochem Cytochem. 1985; 33(8):845-53. DOI: 10.1177/33.8.3894502. View

2.
Coons A . Fluorescent antibody methods. Gen Cytochem Methods. 1958; 1:399-422. View

3.
Long 2nd D, Buggs C . Microwave oven-based technique for immunofluorescent staining of paraffin-embedded tissues. J Mol Histol. 2007; 39(1):1-4. PMC: 3464309. DOI: 10.1007/s10735-007-9093-6. View

4.
Neuberger M . Antibody diversification by somatic mutation: from Burnet onwards. Immunol Cell Biol. 2008; 86(2):124-32. DOI: 10.1038/sj.icb.7100160. View

5.
Lorincz A, Nusser Z . Specificity of immunoreactions: the importance of testing specificity in each method. J Neurosci. 2008; 28(37):9083-6. PMC: 2629537. DOI: 10.1523/JNEUROSCI.2494-08.2008. View