Applications of Proteomics in the Study of Inflammatory Bowel Diseases: Current Status and Future Directions with Available Technologies

Overview

Journal Inflamm Bowel Dis

Publisher Oxford University Press

Specialty Gastroenterology

Date 2008 Oct 11

PMID 18844215

Citations 25

Authors

Philip Alex

Marjan Gucek

Xuhang Li

Affiliations

Soon will be listed here.

Abstract

Inflammatory bowel diseases (IBD) are chronic, heterogeneous, and multifactorial intestinal inflammatory disorders. Major challenges in IBD research include identification of major pathogenic alterations of genes/proteins as well as effective biomarkers for early diagnosis, prognosis, and prediction of therapeutic response. Since proteins govern cellular structure and biological function, a wide selection of proteomic approaches enables effective characterization of IBD pathogenesis by investigating the dynamic nature of protein expression, cellular and subcellular distribution, posttranslational modifications, and interactions at both the cellular and subcellular levels. The aims of this review are to 1) highlight the current status of proteomic studies of IBD, and 2) introduce the available and emerging proteomic technologies that have potential applications in the study of IBD. These technologies include various mass spectrometry technologies, quantitative proteomics (2D-PAGE, ICAT, SILAC, iTRAQ), protein/antibody arrays, and multi-epitope-ligand cartography. This review also presents information and methodologies, from sample selection and enrichment to protein identification, that are not only essential but also particularly relevant to IBD research. The potential future application of these technologies is expected to have a significant impact on the discovery of novel biomarkers and key pathogenic factors for IBD.

Citing Articles

A Current State of Proteomics in Adult and Pediatric Inflammatory Bowel Diseases: A Systematic Search and Review.

Fabian O, Bajer L, Drastich P, Harant K, Sticova E, Daskova N Int J Mol Sci. 2023; 24(11).

PMID: 37298338 PMC: 10253608. DOI: 10.3390/ijms24119386.

The Current Status of Molecular Biomarkers for Inflammatory Bowel Disease.

Alghoul Z, Yang C, Merlin D Biomedicines. 2022; 10(7).

PMID: 35884797 PMC: 9312796. DOI: 10.3390/biomedicines10071492.

Characterization of the intestinal mucosal proteome in cats with inflammatory bowel disease and alimentary small cell lymphoma.

Marsilio S, Droes F, Dangott L, Chow B, Hill S, Ackermann M J Vet Intern Med. 2021; 35(1):179-189.

PMID: 33471936 PMC: 7848303. DOI: 10.1111/jvim.16003.

Systems biology in inflammatory bowel diseases: on the way to precision medicine.

Dovrolis N, Filidou E, Kolios G Ann Gastroenterol. 2019; 32(3):233-246.

PMID: 31040620 PMC: 6479645. DOI: 10.20524/aog.2019.0373.

Application of Proteomics to Inflammatory Bowel Disease Research: Current Status and Future Perspectives.

Assadsangabi A, Evans C, Corfe B, Lobo A Gastroenterol Res Pract. 2019; 2019:1426954.

PMID: 30774653 PMC: 6350533. DOI: 10.1155/2019/1426954.

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