Phospholipase A2 in Human Ascitic Fluid. Purification, Characterization and Immunochemical Detection

Overview

Journal Biochem J

Specialty Biochemistry

Date 1991 Aug 15

PMID 1883335

Citations 2

Authors

P T Kortesuo

T J Nevalainen

Affiliations

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Abstract

A phospholipase A2 (PLA2, EC 3.1.1.4) was purified from human cell-free ascitic fluid (a-PLA2) by ion-exchange chromatography and h.p.l.c. on a reverse-phase column to apparent homogeneity. The enzyme had an Mr of approx. 10,000 as determined by SDS/PAGE. Polyclonal antibodies raised in a rabbit were specific to a-PLA2, as judged by immunoblotting. A time-resolved fluoroimmunoassay (TR-FIA) for measuring the concentration of a-PLA2 in various body fluids was developed. The detection limit of the assay was about 6 ng/ml. The antiserum did not cross-react with pancreatic secretory phospholipase A2 as measured by TR-FIA. The enzyme content was studied in various samples, including normal human serum, buffy-coat leucocytes, synovial fluid, and pancreas and spleen homogenates.

Citing Articles

Rat pancreatic phospholipase A2. Purification, localization, and development of an enzyme immunoassay.

Kortesuo P, Hietaranta A, Jamia M, Hirsimaki P, Nevalainen T Int J Pancreatol. 1993; 13(2):111-8.

PMID: 8501352 DOI: 10.1007/BF02786079.

Distribution of pancreatic (group I) and synovial-type (group II) phospholipases A2 in human tissues.

Nevalainen T, Haapanen T Inflammation. 1993; 17(4):453-64.

PMID: 8406689 DOI: 10.1007/BF00916585.

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