Immunoscreening of Urinary Bladder Cancer CDNA Library and Identification of Potential Tumor Antigen

Overview

Journal World J Urol

Specialty Urology

Date 2008 Oct 2

PMID 18828023

Citations 6

Authors

Ling Chen

Wei Chen

Le Zhao

Hai-Zhen Yu

Xu Li

Affiliations

Soon will be listed here.

Abstract

Objective: To identify potential tumor antigen by immunoscreening the urinary bladder cancer cDNA library with monoclonal antibodies.

Methods: Monoclonal antibodies were prepared. A cDNA expression library was constructed from bladder cancer cell line BLZ211. Immunogenic proteins were identified by immunoscreening the cDNA library with ten monoclonal antibodies.

Results: The cDNA library of BLZ211 cells was established using lambdaZAP as vector. The titer of unamplified cDNA library was 1.39x10(6) pfu/ml with a recombinant rate of 97.72%, and titer of amplified one was 8.4x10(9) pfu/ml. After immunoscreening, ten positive clones representing ten different antigens were identified, which include two proteins with unknown function; coactosin-like 1, eukaryotic translation elongation factor, HNRPA1, histidine triad nucleotide binding protein, KRT7, LCN2, TSTA3, zinc finger protein, C11orf48 and HSPC148.

Conclusion: The cDNA library was of high quality and can be used in further study. By immunoscreening the bladder cancer cDNA library with ten monoclonal antibodies, we identified ten immunogenic proteins that otherwise would not have been identified as potential diagnostic marker and vaccinogens of bladder cancer using the gene discovery effort.

Citing Articles

Study of LBHD1 Expression with Invasion and Migration of Bladder Cancer.

Dong C, Liu Y, Yu G, Li X, Chen L Open Life Sci. 2021; 14:440-447.

PMID: 33817179 PMC: 7874779. DOI: 10.1515/biol-2019-0049.

Non-translational Connections of eEF1B in the Cytoplasm and Nucleus of Cancer Cells.

Negrutskii B Front Mol Biosci. 2020; 7:56.

PMID: 32328499 PMC: 7160314. DOI: 10.3389/fmolb.2020.00056.

Genetic and epigenetic silencing of mircoRNA-506-3p enhances COTL1 oncogene expression to foster non-small lung cancer progression.

Guo S, Yang P, Jiang X, Li X, Wang Y, Zhang X Oncotarget. 2016; 8(1):644-657.

PMID: 27893417 PMC: 5352185. DOI: 10.18632/oncotarget.13501.

Independent overexpression of the subunits of translation elongation factor complex eEF1H in human lung cancer.

Veremieva M, Kapustian L, Khoruzhenko A, Zakharychev V, Negrutskii B, Elskaya A BMC Cancer. 2014; 14:913.

PMID: 25472873 PMC: 4265501. DOI: 10.1186/1471-2407-14-913.

Hsp74, a potential bladder cancer marker, has direct interaction with keratin 1.

Chen L, Wang Y, Zhao L, Chen W, Dong C, Zhao X J Immunol Res. 2014; 2014:492849.

PMID: 25050384 PMC: 4094855. DOI: 10.1155/2014/492849.

References

Landis S, Murray T, Bolden S, Wingo P . Cancer statistics, 1998. CA Cancer J Clin. 1998; 48(1):6-29. DOI: 10.3322/canjclin.48.1.6. View

Okayama H, Berg P . High-efficiency cloning of full-length cDNA. Mol Cell Biol. 1982; 2(2):161-70. PMC: 369769. DOI: 10.1128/mcb.2.2.161-170.1982. View

van Rhijn B, van der Poel H, van der Kwast T . Urine markers for bladder cancer surveillance: a systematic review. Eur Urol. 2005; 47(6):736-48. DOI: 10.1016/j.eururo.2005.03.014. View

Friedl A, Stoesz S, Buckley P, Gould M . Neutrophil gelatinase-associated lipocalin in normal and neoplastic human tissues. Cell type-specific pattern of expression. Histochem J. 1999; 31(7):433-41. DOI: 10.1023/a:1003708808934. View

Nguyen C, Stephen Jones J . Defining the role of NMP22 in bladder cancer surveillance. World J Urol. 2007; 26(1):51-8. DOI: 10.1007/s00345-007-0226-z. View

Madoz-Gurpide J, Lopez-Serra P, Martinez-Torrecuadrada J, Sanchez L, Lombardia L, Casal J . Proteomics-based validation of genomic data: applications in colorectal cancer diagnosis. Mol Cell Proteomics. 2006; 5(8):1471-83. DOI: 10.1074/mcp.M600048-MCP200. View

Zhang L, Chen W, Li X . A novel anticancer effect of butein: inhibition of invasion through the ERK1/2 and NF-kappa B signaling pathways in bladder cancer cells. FEBS Lett. 2008; 582(13):1821-8. DOI: 10.1016/j.febslet.2008.04.046. View

Su T, Suzui M, Wang L, Lin C, Xing W, Weinstein I . Deletion of histidine triad nucleotide-binding protein 1/PKC-interacting protein in mice enhances cell growth and carcinogenesis. Proc Natl Acad Sci U S A. 2003; 100(13):7824-9. PMC: 164672. DOI: 10.1073/pnas.1332160100. View

Lai W, Carballo E, Thorn J, Kennington E, Blackshear P . Interactions of CCCH zinc finger proteins with mRNA. Binding of tristetraprolin-related zinc finger proteins to Au-rich elements and destabilization of mRNA. J Biol Chem. 2000; 275(23):17827-37. DOI: 10.1074/jbc.M001696200. View

10.

Chiu S, Hsieh F, Chen S, Chen C, Shu H, Li H . Clinicopathologic correlation of up-regulated genes identified using cDNA microarray and real-time reverse transcription-PCR in human colorectal cancer. Cancer Epidemiol Biomarkers Prev. 2005; 14(2):437-43. DOI: 10.1158/1055-9965.EPI-04-0396. View

11.

Chiong E, Gaston K, Grossman H . Urinary markers in screening patients with hematuria. World J Urol. 2007; 26(1):25-30. DOI: 10.1007/s00345-007-0229-9. View

12.

Yuan B, Jefferson A, Popescu N, Reynolds S . Aberrant gene expression in human non small cell lung carcinoma cells exposed to demethylating agent 5-aza-2'-deoxycytidine. Neoplasia. 2004; 6(4):412-9. PMC: 1502114. DOI: 10.1593/neo.03490. View

13.

Friedl P, Wolf K . Tumour-cell invasion and migration: diversity and escape mechanisms. Nat Rev Cancer. 2003; 3(5):362-74. DOI: 10.1038/nrc1075. View

14.

Hanai J, Mammoto T, Seth P, Mori K, Karumanchi S, Barasch J . Lipocalin 2 diminishes invasiveness and metastasis of Ras-transformed cells. J Biol Chem. 2005; 280(14):13641-7. DOI: 10.1074/jbc.M413047200. View

15.

Thomas C, Theodorescu D . Molecular markers of prognosis and novel therapeutic strategies for urothelial cell carcinomas. World J Urol. 2006; 24(5):565-78. DOI: 10.1007/s00345-006-0119-6. View

16.

Mathur S, Cleary K, Inamdar N, Kim Y, Steck P, Frazier M . Overexpression of elongation factor-1gamma protein in colorectal carcinoma. Cancer. 1998; 82(5):816-21. DOI: 10.1002/(sici)1097-0142(19980301)82:5<816::aid-cncr3>3.0.co;2-h. View

17.

Oyama M, Kozuka-Hata H, Suzuki Y, Semba K, Yamamoto T, Sugano S . Diversity of translation start sites may define increased complexity of the human short ORFeome. Mol Cell Proteomics. 2007; 6(6):1000-6. DOI: 10.1074/mcp.M600297-MCP200. View

18.

Lai W, Stumpo D, Blackshear P . Rapid insulin-stimulated accumulation of an mRNA encoding a proline-rich protein. J Biol Chem. 1990; 265(27):16556-63. View

19.

Varnum B, Ma Q, Chi T, Fletcher B, Herschman H . The TIS11 primary response gene is a member of a gene family that encodes proteins with a highly conserved sequence containing an unusual Cys-His repeat. Mol Cell Biol. 1991; 11(3):1754-8. PMC: 369492. DOI: 10.1128/mcb.11.3.1754-1758.1991. View

20.

Moll R, Schiller D, Franke W . Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns. J Cell Biol. 1990; 111(2):567-80. PMC: 2116178. DOI: 10.1083/jcb.111.2.567. View