VEGF-mediated Fusion in the Generation of Uniluminal Vascular Spheroids

Overview

Journal Dev Dyn

Publisher Wiley

Specialty Anatomy & Histology

Date 2008 Sep 26

PMID 18816835

Citations 17

Authors

Carmine Gentile

Paul A Fleming

Vladimir Mironov

Kelley M Argraves

W Scott Argraves

Christopher J Drake

Affiliations

Soon will be listed here.

Abstract

Embryonic mouse allantoic tissue (E8.5) was cultured in hanging drops to generate a three-dimensional vascular micro-tissue. The resulting tissue spheroids had an inner network of small diameter vessels expressing platelet endothelial cell adhesion molecule-1 (PECAM-1) and an outer layer of cells expressing SMalphaA, SM22-alpha, and SM-MHC. In a subsequent phase of culture, the fusion-promoting activity of vascular endothelial growth factor (VEGF) was used to transform the inner network of small diameter endothelial tubes into a contiguous layer of cells expressing PECAM-1, CD34, and VE-cadherin that circumscribed a central lumen-like cavity. The blood vessel-like character of the VEGF-treated spheroids was further demonstrated by their physiologically relevant vasodilatory and contractile responses, including contraction induced by KCl and relaxation stimulated by high-density lipoproteins and acetylcholine-induced nitric oxide production.

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