Comparative Studies on Isolated Rat Hepatocytes and AS-30D Hepatoma Cells with Leucocidin from Pseudomonas Aeruginosa

Overview

Journal Naunyn Schmiedebergs Arch Pharmacol

Specialty Pharmacology

Date 1976 Oct 12

PMID 187964

Citations 14

Authors

M Frimmer

J Homann

E Petzinger

U Rufeger

W Scharmann

Affiliations

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Abstract

Leucocidin, one of the cytotoxic principles of Pseudomonas aeruginosa induces potassium loss and swelling in isolated hepatocytes and in AS-30D ascites hepatoma cells in a dose and time dependent manner. Hepatoma cells are more sensitive than normal hepatocytes. As shown by scanning electron microscopy the volume increase of both types of cells is accompanied by disappearance of microvilli. In contrast to phalloidin poisoning no protrusions were formed when the cells were exposed to leucocidin under isotonic conditions.

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References

Weiss E, Sterz I, Frimmer M, Kroker R . Electron microscopy of isolated rat hepatocytes before and after treatment with phalloidin. Beitr Pathol. 1973; 150(4):345-56. DOI: 10.1016/s0005-8165(73)80085-x. View

Berry M, Friend D . High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study. J Cell Biol. 1969; 43(3):506-20. PMC: 2107801. DOI: 10.1083/jcb.43.3.506. View

HEGNER D, PETTER A, Kroker R, Anwer M, Scharmann W, Breuninger V . Leucocidin from Pseudomonas aeruginosa and membrane functions. Naunyn Schmiedebergs Arch Pharmacol. 1976; 293(1):49-55. DOI: 10.1007/BF00498870. View

Frimmer M, Kroker R, Porstendorfer J . The mode of action of phalloidin: demonstration of rapid deformation of isolated hepatocytes by scanning electron microscopy. Naunyn Schmiedebergs Arch Pharmacol. 1974; 284(4):395-8. DOI: 10.1007/BF00504708. View

Knutton S, Sumner M, Pasternak C . Role of microvilli in surface changes of synchronized P815Y mastocytoma cells. J Cell Biol. 1975; 66(3):568-76. PMC: 2109462. DOI: 10.1083/jcb.66.3.568. View