Species-specific Transcription in Mice Carrying Human Chromosome 21

Overview

Journal Science

Specialty Science

Date 2008 Sep 13

PMID 18787134

Citations 169

Authors

Michael D Wilson

Nuno L Barbosa-Morais

Dominic Schmidt

Caitlin M Conboy

Lesley Vanes

Victor L J Tybulewicz

Elizabeth M C Fisher

Simon Tavare

Duncan T Odom

Affiliations

Soon will be listed here.

Abstract

Homologous sets of transcription factors direct conserved tissue-specific gene expression, yet transcription factor-binding events diverge rapidly between closely related species. We used hepatocytes from an aneuploid mouse strain carrying human chromosome 21 to determine, on a chromosomal scale, whether interspecies differences in transcriptional regulation are primarily directed by human genetic sequence or mouse nuclear environment. Virtually all transcription factor-binding locations, landmarks of transcription initiation, and the resulting gene expression observed in human hepatocytes were recapitulated across the entire human chromosome 21 in the mouse hepatocyte nucleus. Thus, in homologous tissues, genetic sequence is largely responsible for directing transcriptional programs; interspecies differences in epigenetic machinery, cellular environment, and transcription factors themselves play secondary roles.

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