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Use of Bordetella Pertussis BP3385 to Establish a Cutoff Value for an IS481-targeted Real-time PCR Assay

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 2008 Sep 12
PMID 18784312
Citations 38
Authors
J L Guthrie
C Seah
S Brown
P Tang
F Jamieson
S J Drews
Affiliations
Soon will be listed here.
Abstract

This study utilized the Bordetella pertussis single-copy PCR target BP3385 as a means of confirming IS481 PCR-positive reactions with cycle threshold (C(T)) values of >35. IS481 PCRs with C(T) values of >35 cycles may represent PCR conditions where there is <1 CFU of B. pertussis per PCR.

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